Development and validation of UV spectrophotometric method for simultaneous estimation of propranolol hydrochloride and rosuvastatin calcium in bulk drug and pharmaceutical dosage form

Namdeo G Shinde, Nagesh H. Aloorkar

Abstract


A new, simple and sensitive UV spectrophotometric method has been developed for simultaneous quantitative determination of propranolol hydrochloride and rosuvastatin calcium in bulk and pharmaceutical dosage form. This is achieved by simultaneous equation (Vierodt’s method) and absorbance ratio (Q- point) method. Propranolol hydrochloride and rosuvastatin calcium exhibits maximum absorbance at 289 nm and 243 nm respectively in methanol as solvent. Beer’s law was found to be obeyed in the concentration range 2-40 µg/ml for propranolol hydrochloride and 2-42 µg/ml or rosuvastatin calcium. Method were validated for linearity, accuracy, precision, LOD, LOQ as per ICH guidelines.


Keywords


Propranolol hydrochloride, Rosuvastatin calcium, Vierodt’s method, absorbance ratio (Q- point) method.

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References


AK Beckett, JB Stenlake. Practical Pharmaceutical Chemistry, 4th edition part II, CBS Publisher and Distributor Pvt. Ltd. 1997: 284-288.

Gaurav Tiwari, Ruchi Tiwari, Brijendra Srivastava, Awani K Rai, KamlaPatha. Simultaneous Estimation of Metronidazole and Amoxicillin in Synthetic Mixture by Ultraviolet Spectroscopy. Asian J. Research Chem. 1(2): Oct.-Dec. 2008: 91-94.

Shah BB, Patel BB, Gohil KN, Patel PM. Difference spectrophotometric method development and validation for simultaneous estimation of rosuvastatin calcium and telmisartan in bulk and combined dosage form. Int Jou of Res in PHAR and Sci. 2 (2); 2012: 106-114.

Jasmine Chaudhari, Aakash Jain, Vipine Saini. Simultaneous estimation of multicomponent formulations by UV visible spectroscopy: An overview. Int Res Jou of Pharmacy. 2 (12); 2011:81-83.

Mohit Rohitas, Abhinav Agrawal, Ashish K Jain, Narendra K Lariya, Anil K Kharya and Govind P Agrawal.Development of simultaneous spectrophotometric method of mesalazine and prednisolone in same dosage form. Int J Appl Pharm, 2 (4); 2010: 8­11.

PY Pawar, Ankita R, BhagatSonu R. Lokhande and Amruta A Bankar. Simultaneous estimation of atorvastatin calcium and aspirin in pure and capsule dosage form by using U.V. spectrophotometric method. Der PharmaChemica, 5(3); 2013: 98-103.

SowjanyaGummadi, Devi Thota. Development and validation of UV spectroscopic methods for simultaneous estimation of ciprofloxacin and tinidazole in tablet formulation. International Current Pharmaceutical Journal, 1(10); 2012: 317-321.

Mallikarjuna Rao N, GowriSankar D. Simultaneous estimation of s (-) amlodipine and hydrochlorothiazide in bulk and tablet dosage form by simultaneous equation method. International Journal of Pharmacy and Pharmaceutical Science Research, 1 (1); 2011:1-5.

Alka Gupta, P. Mishra, K. Shah. Simpl UV spectrophotometric determination of rosuvastatin calcium in pur form and in pharmaceutical formulation. E-Journal of Chemitry, 6 (1); 2009: 89-92.


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