Simultaneous determination of Cefixime trihydrate and Ofloxacin in pharmaceutical dosage form by second order derivative UV spectrophotometry

Audumbar Digambar Mali, Ritesh Bathe, Ashpak Tamboli


 Derivative spectrophotometry offers a useful approach for the analysis of drugs in multi-component formulation. In this study a second order derivative spectrophotometric method is applied for the simultaneous determination of Cefixime Trihydrate and Ofloxacin in Tablet dosage form. The measurements were carried out at wavelengths of 307 and 298 nm for Cefixime Trihydrate and Ofloxacin respectively. The method was found to be linear (r²=0.999) in the range of 4-20 μg/ml for Cefixime Trihydrate in the presence of 20 μg/ml of Ofloxacin at 307 nm. The linear correlation (r²=0.999) was obtained in the range of 4-20 μg/ml for Ofloxacin in the presence of 20 μg/ml of Cefixime Trihydrate at 298 nm. The method was successfully used for simultaneous determination of Cefixime Trihydrate and Ofloxacin in tablet dosage form without any interference from excipients and prior separation.


Cefixime Trihydrate, Ofloxacin, UV visible spectrophotometry, Method Validation, Second order derivative method.

Full Text:



Suddhasattya Dey, Prasanna Kumar Pradhan, U. M. Upadhayay, Shreya Shah, Kuntal Goswami. UV Spectrophotometric Determination of Cefixime in Bulk and its Dosage Form. Journal of Pharmacy Research. 2012; 5.12: 5419-5422.

Shreya R.Shah, Prasanna Pradhan, Suddhasattya Dey. Quantitative Estimation of Cefixime and Moxifloxacin in Pharmaceutical Preparation by Uv Spectrophotometric Method. International Journal of Pharmtech Research. 2013; 5.1: 198-204.

Md. Ahasan Ullah Nayon, Jeb-Un Nesa, Md. Nasir Uddin, Md. Shah Amran, Umme Bushra. Development and Validation of UV Spectrometric Method for the Determination of Cefixime Trihydrate in Bulk and Pharmaceutical Formulation. Asian Journal of Biomedical and Pharmaceutical Sciences.2013; 3.22:1-5.

Mahesh Attimarad, Anroop B. Nair. Simultaneous Determination of Ofloxacin and Cefixime by First and Ratio First Derivative UV Spectrophotometry. Chronicles of Young Scientists. 2011; 2.3: 144-149.

Arshad HM, Gauhar S, Bano R, Muhammad IN. Development of HPLC-UV method for analysis of cefixime in raw materials and capsule. Jordan journal of pharmaceutical sciences. 2009; 2.1: 53-65.

Vijaya Vichare, Vrushali Tambe, S V Joshi, S N Dhole. New Simultaneous UV-Visible Spectrophotometric Methods for Estimation of Ofloxacin and Ketorolac Tromethamine in Ophthalmic Dosage Form. Asian J. Pharm. Ana. 2013; 3.2: 53-57.

Sakane K, Kawabata K, Inamoto Y, Yamanaka HT. Research and development of new oral cephems, cefixime and cefdinir. Yakugaku Zasshi.1993; 113:605-26.

Anbarasi B, Seetharaman R, Suresh V, Senthilkumar, Parimaladevi N. Analytical method development and validation of simultaneous RP- HPLC estimation of ornidazole and Ofloxacin in tablet dosage form. J Pharm Res. 2011; 4.1:272-273.

Kapil S. Khandagle, Santosh V. Gandhi, Padmanbh B. Deshpande and Nilesh V.Gaikwad. A simple and sensitive RP-HPLC for simultaneous estimation of cefixime and ofloxacin in combined tablet dosage form. International journal of pharmacy and pharmaceutical sciences. 2011; 3.1: 46-48.

Dhoka MV, Sandage SJ, Dumbre SC. Simultaneous Determination of Cefixime Trihydrate and Dicloxacillin Sodium in Pharmaceutical Dosage Form by Reversed-Phase High-Performance Liquid Chromatography. J AOAC Int. 2010; 93: 531-5.

Prabhu S, Vijay Amirtharaj R, Senthilkumar N. Simultaneous RP-HPLC method development and validation of cefixime and ofloxacin in tablet dosage form. Asian J Res Chem 2010; 3:367-369.

Khandagle KS, Gandhi SV, Deshpande PB, Kale AN, Deshmukh PR. High performance thin layer chromatographic determination of cefixime and ofloxacin in combined tablet dosage form. J Chem Pharm Res 2010; 2:92-96.

Nevin E. Application of derivative-differential UV spectrophotometry and ratio derivative spectrophotometric determination of mephenoxalone and acetaminophen in combined tablet preparation. J. Pharm. and Biomed. Ana.1999; 21: 429–437.

Mohabbat Ullah, Anisur Rahman and Saugata Dutta. Development and Validation of A Stability Indicating RP-HPLC Method for Determination of Flucytosine and its Process Related Impurities in Injectable Pharmaceuticals. International Journal of Advances in Pharmaceutical Analysis. 2015; 5.1: 1-9.

International Conference on Harmonization (ICH) of Technical Requirements for the registration of Pharmaceuticals for Human use, Validation of Analytical Procedures Methodology. ICH-Q2 (R1), Geneva. 1996, 1-8.


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


  • There are currently no refbacks.

Copyright (c) 2015 International Journal of Advances in Pharmaceutical Analysis

Most read articles by the same author(s)