Simultaneous determination of Propranolol hydrochloride and Hydrochlorothiazide in Tablets formulation using spectrophotometric technique (Simultaneous Equation Method)

Bhadresh V Savaj, Hasumati A. Raj, Rajanit V Sojitra, Paras Virani


A new spectrophotometric method was developed for simultaneous determination of compounds with interfering spectra in binary mixtures without previous separation, showing significant advantages over the conventional methods regarding minimal data manipulation and applicability. The proposed method was applied for the determination of Propranolol hydrochloride and Hydrochlorothiazide in Tablets formulation, for determination of sampling wavelength, 10 μg/ml of each of PRO and HCT were scanned in 200-400 nm range and sampling wavelengths were 289 nm for PRO and 270 nm for HCT are selected for development and validation of simultaneous equation method. For this method linearity observed in the range of 10-50 μg/ml for PRP and 5-25 μg/ml for HCT, and in their pharmaceutical formulation with mean percentage recoveries 100.13± 0.86 and 100.07± 0.58, respectively. The method was validated according to ICH guidelines and can be applied for routine quality control testing.


Spectroscopic method, simultaneous equation method, propranolol hydrochloride and hydrochlorothiazide.

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S. Altinos, D.J. Tekeli. Analysis of glimepiride by using derivative UV spectrophotometric method, Pharm. Biomed. Anal., 2011, 24: 507.

J. Chaudhary, A. Jain, V. Saini, Analytical Method Development and Validation for the Simultaneous estimation of Alprazolam and Propranolol in their combined dosage form. Int. J. Drug Delivery., 2012, 4: 310-315.

S. J. Daharwal, Development and Validation of UV Spectrophotometric Method for

Simultaneous Estimation of Diazepam and Propranolol in Bulk Drug and Its Formulations. Asian J. Pharm. Ana., 2013, 3: 20-23.

M. Suman, N.C. Rao, S. K. Kumar, P. Venkateswarlu, Development of Simple Spectrophotometric Method for the Determination of Propranolol using p-Chloranilic acid in Bulk and Dosage forms. Res. J. Pharm. Bio. Chem. Sci., 2013, 4: 342-346.

N.A. Shivarkar, P.B. Dudhe, M.A. Nagras, K.S. Jain, Simultaneous Estimation of Flunarizine dihydrochloride and Propranolol hydrochloride in Bulk Drug and Capsule. Int. J. ChemTech Res., 2012, 4: 1007-1012.

K.S. Rao, M.Panda, N.Keshar, Spectrophotometric methods for the simultaneous estimation of losartan potassium and hydrochlorothiazide in tablet dosage forms. Chronicles of Young Scientists., 2011, 2: 155-159.

N.S. Abdelwahab, Spectrophotometric methods for simultaneous determination of Carvedilol and Hydrochlorothiazide in combined dosage form. Arabian J. Chem., 2011, (in press)

M.C.F. Ferraro, P.M. Castellano, T.S. Kaufman, Simultaneous determination of amiloride hydrochloride and hydrochlorothiazide in synthetic samples and pharmaceutical Formulations by multivariate analysis of spectrophotometric data. J. Pharm. Biomed. Anal., 2002, 30: 1121-1131.

T.S.Belala, N. Hoda, G. Daabeesb, M.A. Khalekb, M.S.Mahrousb, M.M.Khamis, New simple spectrophotometric method for determination of the binary mixtures (atorvastatin calcium and ezetimibe; candesartan cilexetil and hydrochlorothiazide) in tablets. J. Pharm. Anal., 2013, 3: 118–126.

N.M. Bhatia, R.B. Desai, S.D. Jadhav, simultaneous estimation of losartan potassium and hydrochlorothiazide from tablets by first order derivative spectroscopy. Int. J. Pharm Pharm. Sci., 2013, 5: 231-235.

V.R. Galande, K.G. Baheti, S. Indraksha, M. H. Dehghan, Estimation of Amlodipine Besylate, Valsartan and Hydrochlorothiazide in Bulk Mixture and Tablet by UV Spectrophotometry. Indian J. Pharm. Sci., 2012,74: 18-23.

Indian Pharmacopoeia, the Indian Pharmacopoeia Commission, Ghaziabad, Govt. of India Ministry of Health and Family Welfare, 2010, vol. III, 1987.

K.D. Tripathi , Essentials of Medical Pharmacology, Sixed Ed., Jaypee Brother’s Medical Publishers (P) LTD, st Louis, 2008.

Indian Pharmacopoeia, the Indian Pharmacopoeia Commission, Ghaziabad, Govt. of India Ministry of Health and Family Welfare, 2010, vol. II, 1451.

H. Lüllmann, L. Hein, K. Mohr D. Bieger, Color Atlas of Pharmacology, Third Ed., Thieme Stuttgart publication, 2005.


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