Zero Order and Area Under Curve Spectrophotometric methods for determination of Oxcarbazepine in Pharmaceutical Formulation

Santosh Balaso Jadhav, Nilesh Patil, Ashpak Tamboli

Abstract


Simple, fast and reliable spectrophotometric methods were developed for determination of Oxcarbazepine in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Distilled Water. The quantitative determination of the drug was carried out using the zero order derivative values measured at 256 nm and the area under the curve method values measured at 252-258 nm (n=2). Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Oxcarbazepine using 5-25 μg/.ml (r²=0.998 and r²=0.9986) for zero order and area under the curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, precise and sensitive to assay of Oxcarbazepine in tablets.


Keywords


Oxcarbazepine, UV visible spectrophotometry, AUC, Method Validation

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References


O’Neil MJ. 2001, Merck Index, an Encyclopedia of Chemicals,Drugs, and Biologicals. 13th ed. Whitehouse Station, NJ; Merck & Co., Inc.

Murray L. 2002, Physician's Desk Reference. 56th ed. Thomson Health Care; P. 2407.

Rao KS, Calvo MEB (2007) Determination of oxcarbazepine by Square Wave Adsorptive Stripping Voltammetry in pharmaceutical preparations. J Pharm Biomed Anal 43: 1156-1160.

Fortuna A, Alves G, Anabela A, Lopes B, Falcão A, et.al. (2012) A chiral liquid chromatography method for the simultaneous determination of oxcarbazepine, eslicarbazepine, R-licarbazepine and other new chemical derivatives BIA 2–024, BIA 2–059 and BIA 2–265, in mouse plasma and brain. Biomed Chromatogr 26: 384-392.

Levert H, Odou P, Robert H (2002) LC determination of oxcarbazepine and its active metabolite in human serum. J Pharm Biomed Anal 28: 517-525.

Pucci V, Kenndler E, Raggi MA (2003) Quantiation of oxcarbazepine and its metabolites in human plasma by micellarelectrokinetic chromatography. Biomed Chromatogr 17: 231-238.

Von Unruh GE, Paar WD (1986) Gas chromatographic/mass spectrometric assays for oxcarbazepine and its main metabolites, 10-hydroxy-carbazepine and carbazepine-10, 11-trans-diol. Biomed Environ Mass Spectrom 13: 651-656.

Rao KS, Belorkar N, MEB Rao (2009) Development and validation of stability-indicating liquid chromatographic method for the quantitative determination of oxcarbazepine in tablet dosage forms. Pharm Anal 1: 270-277.

Mandrioli R, Ghedini N, Albani F, Kenndler E, Raggi MA (2003). Liquid chromatographic determination of oxcarbazepine and its metabolite in plasma of epileptic patients after solid-phase extraction. J Chromatogr B AnalytTechnol Biomed Life Sci 783: 253-263.

Contin M, Balboni M, Callegati E, Candela C, Albani F, et al. (2005). Simultaneous liquid chromatographic determination of lamotrigine,oxcarbazepinemonohydroxy derivative and felbamate in plasma of patients with epilepsy. J Chromatogr B AnalytTechnol Biomed Life Sci 828: 113-117.

Mazzucchelli I, Franco V, Cinzia F, Roberto M, Emilio P, et al. (2007) a Novel EnantioselectiveMicroassay for the High-Performance Liquid Chromatography Determination of Oxcarbazepine and Its Active Metabolite Monohydroxycarbazepine in Human PlasmaTher Drug Monit 29: 319-324.

Kimiskidis V, Spanakis M, IoannisNiopas, DimitriosKazis, ChrysiGabrieli, et al. (2007) Development and validation of a high performance liquid chromatographic method for the determination of oxcarbazepine and its main metabolites in human plasma and cerebrospinal fluid and its application to pharmacokinetic study. J Pharm Biomed Anal 43: 763-768.

Chi DY, Li ZD (2006) YaowuFenxiZazhi 26: 1195-1198.

Klys M, Rojek S, Bolechała F (2005) Determination of oxcarbazepine and its metabolites in postmortem blood and hair by means of liquid chromatography with mass detection (HPLC/APCI/MS). J Chromatogr B: Anal Technol Biomed Life Sci. 825: 38-46.

Mandrioli R, Ghedini N, Albani F, Kenndler E, Raggi MA. (2003) Liquid chromatographic determination of oxcarbazepine and its metabolite in plasma of epileptic patients after solid-phase extraction. J Chromatogr B: Anal Technol Biomed Life Sci 783: 253-263.

Rouan MC, Decherf M, Le Clanche V, Lecaillon JB, Godbillon J (1994) Automated microdialysis of oxcarbazepine and its monohydroxy and transdiol metabolites in plasma by liquid chromatography. J Chromatogr B: Biomed Appl 658: 167-172.




DOI: http://dx.doi.org/10.7439/ijasr.v1i3.1780

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