Novel Anti-Diabetic Formula: Recover Islet β-Cell dysfunction with the implementation of molecular approach

Susmit Kosta, CBS Dangi, Chander Shekhar, Manpreet Kaur, S N Malviya, Mohan Tiwari, Sabiha K. Khan, Shruti Kapoor


The regulation of PPARα and PPARγ at m-RNAs expression level is responsible for Islet β-cell dysfunction and insulin secretion seen in diabetic rats. Many traditional treatments have been recommended in the alternative system of medicine for the treatment of diabetes mellitus. Stevia rebaudiana and Gurmmar is a nontoxic natural therapeutic agent revealed to own diuretic, hypotensive and hypoglycemic properties. The ground of this study was to examine the anti-hyperglycemic property, expression of m-RNA at PPARα and PPARγ receptors and islet β-cells dysfunction of a combined aqueous extracts of Stevia rebaudiana and Gurmmar is on streptozotocin induced diabetic wistar rats. The diabetes induced rats were fed with plant extracts at the increasing dosage of 200mg, 300mg and 500mg of body weight. The combined plant extracts administrated rats revealed a significantly (P<0.001) rise of serum insulin levels and higher reduction in hyperglycemia or hyperlipidemia when compared to the diabetic control rats (P<0.001) and also an increase the m-RNAs expansion of PPARα and PPARγ respectively. The histological studies of the endocrine region of pancreas of diabetic rats revealed that shrinkage of β-cells of islets of langerhans. The combined plant extracts treated rats revealed recovery of β-cells. The recovery of β-cells was evident at higher dose level i.e. 500mg/by wt extracts fed groups. According to the biochemical, molecular and histological results obtained, it was concluded that the combined plant extracts of this plant may sustain anti-diabetic properties by recovery Islet β-cell dysfunction with the implementation of molecular approach.


Stevia rebaudiana ,Gurmmar, PPARα and PPARγ

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