Preliminary phytochemical screening and cytotoxic potentials from leaves of Sanchezia speciosa Hook.f

Shumaia Parvin, Md. Abu Shuaib Rafshanjani, Md. Abdul Kader, Tahmida Sharmin

Abstract


Background:Sanchezia speciosa Hook.fis an important medicinal plant with several ethnomedicinal properties. In the present experiment the leaves of this plant were screened for the presence of major phytochemical groups. The phytochemicals are the wide variety of compounds produced by plants manipulated widely in the pharmacognostic drug development and in the treatment of the major ailments.

Methods:Phytochemical screenings were performed by various functional group tests. All the leaves extracts were subjected to brine shrimp lethality bioassay and standard anticancer drug vincristine sulphate was used as positive control.

Results:Phytochemical investigation of the leaves extracts showed the presence of alkaloids, glycosides, flavonoids, triterpenoids, carbohydrates, steroids, phenolic compounds, saponins and tannins. The ethyl acetate fractionates of the leaves extracts were positive for all the phytochemicals tested while n-hexane fractionates were negative for flavonoids and phenolic compounds. The n-hexane and ethyl acetate soluble fractionates showed significant cytotoxic activities with median lethal concentrations (LC50) 19.95 µg/ml and 12.88µg/mlwhile LC50 for the vincristine sulphate was 10.96 µg/ml.

Conclusion: This study shows that the leaves of Sanchezia speciosa Hook.fmay have potentials in control of carcinogenesis in the field of pharmacology.


Keywords


Sanchezia speciosa Hook.f, phytochemicals, Artemia salina Leach, brine shrimp lethality test

Full Text:

PDF

References


Sheu JR, Jayakumar T, Chang CC, Chen YC, Priya S, Ong ETet al. Pharmacological actions of an ethanolic extracts of the leaves of Hemigraphis colorata and Clerodendron phlomoides. Clin Mole Med2012; 3(1): 1-3.

Rates SMK. Plants as source of drugs. Toxicon2001;39: 603-613.

Nyarko HD, Barku VYA and Batama J. Antimicrobial examinations of Cymbopogoncitratus and Adiatum capillus-veneris used in Ghanajan folkloric medicine. Int J Life Sci Pharma Res 2012;2(2):115-121.

Momin MAM, Habib MR, Hasan MR, Nayeem J, Uddin N and Rana MS.Anti-inflammatory, antioxidant and cytotoxicity potential of ethanolic extract of two Bangladeshi bean Lablab purpureous (L.) sweet white purple. Int J PharmSci Res2012; 3(3): 776-781.

Hill AF. Economic Botany. A Text Book of Useful Plants and Plant Products. 2nd ed. New York: Mcgraw-Hill book company Inc;1952.

McLaughlin JL, Chang CJ and Smith DL. Simple bench-top bioassay (brine shrimp and potato discs) for the discovery of plants’ antitumor compounds. In: Kinghorn AD, Balandrin MF, editors. Human Medicinal Agents from Plants. Washington: ACS Symposium 534, American Chemical Society; 1993. p. 112-137.http://dx.doi.org/10.1021/bk-1993-0534.ch009.

Moshafi MH, Sharififar F, Dehghan GR and Ameri A. Bioassay screening of the essential oil and various extracts of fruits of Heracleum persicum desf. and rhizomes of Zingiber officinale rosc. using brine shrimp cytotoxicity assay. Iran J PharmRes2009; 8(1): 59-63.

Carballo JL, Hermandez-inda ZL, Perez P and Garcia-Gravalos MD. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotechnology2002; 2 (17). http://dx.doi.org/10.1186/1472-6750-2-17.

Leonard EC and Smith LB. Sanchezia and related American Acanthaceae. Rhodora1964; 66: 313-343.

Ahmed ZU. Encyclopedia of Flora and Fauna of Bangladesh.1st ed. Vol 6, Dhaka, Nimtali: Asiatic Society of Bangladesh; 2008,p. 59.

Trease EG and Evans WC. Textbook of Pharmacognosy. 14th ed. U.K: W.B. Saunders Company; 1997. p. 119.

Jeffery GH, Bassett J, Mendham J, et al. Vogel’s Textbook of Quantitative Chemical Analysis. 5th ed.England: Longman Group UK Ltd; 2000. p.161.

Van Wagenen BC, Larsen R, Cardellina II JH, Randazzo D, Lidert ZC and Swithenbank C. Ulosantoin, a potent insecticide from the sponge Ulosaruetzleri.J Organic Chem1993; 58: 335-337.

Kokate CK. A Text Book of Practical Pharmacognosy. 5th ed. Vallabh Prakashan;2005. p. 107-111.

Tiwari P. Kumar B, Kaur M, Kaur G and Kaur H. Phytochemical screening and extraction: A review. Int Pharm Sci 2011; 1:103-104.

Mamta S. and Jyoti S. Phytochemical screening of Acorus calamus and Lantana camara. Int Res J Pharm2012; 3(5): Pages 324-326.

Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nicholas DE and McLaughlin JL.Brine Shrimp: A convenient general bioassay for active plants constituents. PlantaMedica1982; 45(5): 31-34. http://dx.doi.org/10.1055/s-2007-971236.

Khan, A., Rahman, M. and Islam, M.S. (2008). Antibacterial, antifungal and cytotoxic activities of amblyone isolated from Amorphophalus campanulatus. Indian J Pharmacol 40(1): 41-44. http://dx.doi.org/10.4103/0253-7613.40489.

Parvin S, Kader MA, Rahman MA, Wahed MII and Haque ME. Antibacterial activities and brine shrimp lethality bioassay of the chloroform extract of stem bark of Crataeva nurvala Buch Ham. Int J Pharm Sci Res2012; 3(3): 830-834.

Hossain MS, Hossain MA, Islam R, Alam AHMK, Kudrat-e-Zahan Sarkar S and FarooqueMA. Antimicrobial and cytotoxic activities of 2-aminobenzoic acid and 2- aminophenol and their coordination complexes with Magnesium (Mg-II). Pak J Bio Sci2004; 7(1): 25-27.

Ara N, NurMH, Amran MS, Wahid MII and Ahmed M.In vitro antimicrobial and cytotoxic activities of leaves and flowers extracts from Lippia alba. Pak JBio Sci2009; 12(1): 87-90.

Starmans DA and Nijhuis HH. Extraction of secondary metabolites from plant material: a review. Trends in Food Sci Technol 1996; 7(6):191-197. http://dx.doi.org/10.1016/0924-2244(96)10020-0

Sharma R, Shrivastava R, Mishra J and Dubey S. Preliminary phytochemical screening of Lantana camara Linn. J Chem Bio and Physical Sci2013; 3(4): 2836-2839.

Peteros NP and Uy MM. Antioxidant and cytotoxic activities and phytochemical screening of four Philippine medicinal plants. J Med Plant Res2010; 4(5): 407-414.

Olila D, Opuda-Asibo J and Olwa-Odyek. Bioassay-guided studies on the cytotoxic and in vitro trypanocidal activities of a sesquiterpene (Muzigadial) derived from a Ugandan medicinal plant (Warburgia ugandensis). African Health Sci2001; 1(1): 12-15.

AlmeidaPA, Silva TMS and Ecxhevarria A.Mesoionic 5-alkyl-5, 3-dithiolium-4-thiolates; synthesis and brine shrimp toxicity. Heterocyclic Communications2002; 8(6): 593-600.

Nino J, Narvaez DM, Mosquera OM and Correa YM. Antibacterial, antifungal and cytotoxic activities of eight Asteraceae and two Rubiaceae plants from Colombian biodiversity. BrazilJ Microbiol2006; 37: 566-570.

Paydar MJ, Wong YL, Moharam BA, Wong WF and Looi CY. In vitro anti-oxidant and anti-cancer activity of methanolic extract from Sanchezia speciosa leaves. PakJ Bio Sci2013; 16(20): 1212-1215.http://dx.doi.org/10.3923/pjbs.2013.1212.121 5

Rafshanjani MAS, Parvin S, Kader MA, SahaMR, AkhtarMA.In vitro antibacterial, antifungal and insecticidal activities of ethanolic extract and its fractionates of Sanchezia speciosa Hook.f.Int Res J Pharm2014; 5(9): 717-720. http://dx.doi.org/10.7897/2230-8407.0509146




DOI: http://dx.doi.org/10.7439/ijasr.v1i3.1842

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.


Copyright (c) 2015 International Journal of Advances in Scientific Research

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.