Estimation of Ciprofloxacin Hydrochloride in Bulk and Formulation by Derivative UV-Spectrophotometric Methods

Rekha Kharat, Santosh Jadhav, Dilshadbee Tamboli, Ashpak Tamboli


Simple, fast and reliable spectrophotometric methods were developed for determination of Ciprofloxacin Hydrochloride in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Distilled Water. The quantitative determination of the drug was carried out using the zero/0th, first, and second order method values measured at264, 273and 273 nm respectively. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ciprofloxacin Hydrochloride using 2-10μg/ml (r²=0.9991, r²=0.9993, r²=0.9955) for zero, first and second order spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ciprofloxacin Hydrochloride in pharmaceutical formulations.


Ciprofloxacin Hydrochloride, UV visible spectrophotometry, Zero, first and second order derivative spectrum

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