Estimation of Ofloxacin in Bulk and Formulation by Second Order DerivativeUV-Spectrophotometric Methods

Shivaji Shinde, Santosh Jadhav, Rekha Kharat, Afaque Ansari, Ashpak Tamboli

Abstract


Simple, fast and reliable spectrophotometric methods were developed for determination of Ofloxacin in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 295-301nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ofloxacin using 2-10μg/ml (r²=0.9947) for second order Derivative Area under Curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ofloxacin in pharmaceutical formulations.


Keywords


Ofloxacin, Second order Derivative, Area under Curve (AUC).

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References


Budavari S. Eds. In. The Merck Index. Merck & co.,Inc; Whitehouse Station, NJ., 2001, 1213 and 1229.

British pharmacopoeia. Licensing division HMSO, Norwich, 2003, 357.

United States Pharmacopoeia. United States Pharmacopoeia Convention, Inc. Rockville, 2004, 1335.

European Pharmacopoeia. EDQM, Council of Europe,Strasbourg, France. 2005, 5th Ed, 2131.

Hesham Salem, American Journal of Applied Sciences, 2005, 2, 719- 729.

Mathur SC, Kumar Y, Murugesan N, Rathode YKS and Sethi PD, Indian drugs, 1992, 29, 376-377.

Bhusari KP* and Chaple DR., Asian J. Research Chem 2009, 2(1), 60-62

Saumil Mehta, Sukhdev Singh, KishorChikhalia,Vishal Shah, GirishSaraswat, International Journal of PharmTech Research, 2012,4,(3) 975-985.

Arjekar AP, Kapadia US, Raj SV and Kunjir SS, Indian Drugs, 1996, 33, 261-266.

SilveriaEvL and Schapoval, EES, J. Pharm .Biomed. Anal, 2002, 1-2, 91-96.

Shiga, T., Shiga, K. and Kuroda, M., Detection of tallow adulteration in cow ghee by Derivative Spectrophotometry, Anal. Biochem, 44, 291, 1971.

International Conference on Harmonization (ICH) of Technical Requirements for the registration of Pharmaceuticals for Human use, Validation of Analytical Procedures Methodology; ICH-Q2(R1), Geneva, pp.1-8, 1996.




DOI: http://dx.doi.org/10.7439/ijasr.v1i5.1977

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