Estimation of Ampicillin trihydrate in bulk and formulation by first order derivative area under curve UV-spectrophotometric methods

Audumbar Digambar Mali, Ritesh Bathe, Ashpak Tamboli


Simple, fast and reliable spectrophotometric methods were developed for determination of Ampicillin Trihydrate in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 224-231nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ampicillin Trihydrate using 5-25μg/ml (r²=0.997) for first order Derivative Area under Curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ampicillin Trihydrate in pharmaceutical formulations.


Ampicillin Trihydrate, First order Derivative, Area under Curve (AUC)

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