Phytochemical and in-vitro antioxidant evaluation of methanolic and aqueous extract of Lepidagathis cuspidata

Nilesh P. Babre, T. Shivraj Gouda, Narayanswamy Lachmanan Gowrishankar

Abstract


The objective of present study was to evaluate preliminary phytoconstitutents and in-vitro antioxidant potential of   Lepidagathis cuspidata. Methanolic and aqueous extract of aerial part of plants was prepared and tested for the presence of different phytoconstitutents. The in-vitro antioxidant potential of extract were evaluate by different in-vitro methods. TLC of extracts were performed by using various solvent systems. The present study revealed that the MELC and AELC contained flavonoids, glycosides, phenols, saponins, steroids and terpenoids However, alkaloids were detected only in MELC. Tannins were absent in methanolic as well as in aqueous extracts.

The in-vitro antioxidant potential MELC and AELC was evaluated by DPPH and FRAP. In both methods MELC possesses good antioxidant activity as compared to AELC. Ascorbic acid is used as a standard.


Keywords


Lepidagathis Cuspidata, Thin Layer Chromatography, Antioxidant activity, DPPH, FRAP

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DOI: http://dx.doi.org/10.7439/ijbar.v8i11.4516

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