DNA damage in a patient with Systemic Lupus Erythematosus and Nephropathy- A Case Report

Gurleen Kaur Tung, Gursatej Gandhi

Abstract


Objectives:

Systemic Lupus Erythematosus (SLE) is a complex, multisystem autoimmune inflammatory disease. The inflammatory response in SLE exacerbates oxidative stress which tends to promote lipid peroxidation, protein oxidation and damage to DNA. Documentation of manifestation of DNA damage in SLE patients is scarce and related studies have not come to attention from this part of the world, therefore the present study showcases a case report on SLE.

Methods:

Assessment of DNA damage in peripheral blood leukocytes and oxidative stress biomarkers in blood sera of a 12y-old male presenting with SLE and nephropathy (on dialysis therapy) was made.

Results:

Basal DNA damage in PBL scored as percent DNA in tail was higher (51.48%) compared to levels (40.38%) in an age-and sex-matched healthy control. Quantitative measures of DNA damage revealed DF of 96 vs. 94 and DI of 317 vs. 208 in the case and the control, respectively. Serum lipid peroxidation as estimated from MDA level was 3x higher (1.759µmol/l) compared to its level in a healthy control (0.571µmol/l). OSI was slightly higher in the patient (0.134 arbitrary units) compared to that in the control (0.132 arbitrary units). The atherogenic indices were higher in the present case compared to ratios in the control.

Conclusion:

The results from the case report on increased oxidative stress, dyslipidemia, genetic damage and atherogenic indices support observations from earlier studies and imply onset of other complications in patients with SLE. The assessment of various blood-based biomarkers (as carried out in the present case) in SLE patients may assist in disease diagnosis, prognosis and optimal disease management.


Keywords


Genetic damage, lupus nephritis, oxidative stress

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References


Pons-Estel GJ, Alarcón GS, Scofield L, Reinlib, L, Cooper GS. Understanding the epidemiology and progression of systemic lupus erythematosus. Semin Arthritis Rheum 2010; 39:257.

Malaviya AN, Singh RR, Singh YN, Kapoor SK, Kumar A. Prevalence of systemic lupus erythematosus in India. Lupus 1993; 2:115-8.

Saadany HE, Sergany ME, Kasem E, Batch MM, Zakaria SS, Mourad H, Moustafa T. Biochemical and genetic risk factors for atherosclerosis in systemic lupus erythematosus. The Egyptian Rheumatologist 2011; 33:35–43.

Rahman A, Isenberg DA. Systemic lupus erythematosus. N Engl J Med 2008; 358:929–39.

Iacconi C, Bulleri A, Tavoni A, Iacconi P, Bombardieri S, Caramella D. Patient With Systemic Lupus Erythematosus (SLE), Complex Adnexal Masses And Ascites. Brit J Radiol 2005; 4.

Cucchiari D, Graziani G, Ponticelli C. The dialysis scenario in patients with systemic lupus Erythematosus. NDT 2013; 0:1–8.

Huang JW, Hung KY, Yen CJ, Wu KD, Tsai TJ. Systemic lupus erythematosus and peritoneal dialysis: outcomes and infectious complications. Periton Dialysis Int 2000; 21:143–47.

Cheigh JS, Stenzel KH. End-stage renal disease in systemic lupus erythematosus. AJKD 1993; 21:2-8.

Shah D, Mahajan N, Sah S, Nath SK, Paudyal B. Oxidative stress and its biomarkers in systemic lupus erythematosus. J Biomed Sci 2014; 21:1-13.

Zhang Q, Ye D, Chen G, Zheng Y. Oxidative protein damage and antioxidant status in systemic lupus erythematosus. Clin Exp Dermatol 2010; 35: 287-94.

Kurien B, Scofield R. Free radical mediated peroxidative damage in systemic lupus erythematosus. Life Sci 2003; 73: 1655-66.

Hassan S, Gheita T, Kenawy S, Fahim A, El-sorougy I, Abdou M. Oxidative stress in systemic lupus erythematosus and arthritis patients: Relationship to disease manifestations and activity. Int J Rheum Dis 2011; 14:325-31.

Montalvão TM, Miranda-Vilela AL, Roll MM, Grisolia CK, Santos-Neto L. DNA damage levels in systemic lupus erythematosus patients with low disease activity: An evaluation by comet assay. Advances in Bioscience and Biotechnology 2012; 3: 983-8.

Schupp N, Stopper H, Rutkowski P, Kobras K, Nebel M, Bahner U, Vienken, J, Heidland A. Effect of different hemodialysis regimens on genomic damage in end-stage renal failure. Semin Nephrol 2006; 26:28–32.

http://nephron.com/cgi-bin/CGSI.cgi

Kumar N, Shekhar C, Kumar P, Kundu AS. Kuppuswamy's socioeconomic status scale-updating for 2007. Indian J Pediatr 2007; 74: 1131-2.

Khadilkar VV, Khadilkar AV, Borade AB, Chiplonkar SA. Body Mass Index Cut-offs for Screening for Childhood Overweight and Obesity in Indian Children. Indian Pediatr 2010; 49: 29-34.

Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175:184-91.

Ahuja YR, Saran R. Potential of Single Cell Gel Electrophoresis Assay (Comet Assay) in Heavy Ion Radiation Biology. Int J Hum Genet 2001; 1:151-56.

Nadin SB, Roig LMV, Ciocca DR. A Silver Staining Method for Single-cell Gel Assay. J Histochem Cytochem 2001; 49: 1183–86.

Noroozi M, Angerson WJ, Lean MEJ. Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes. Am J Clin Nutr 1998; 67:1210–18.

Franke SIR, Prá D, daSilva J, Erdtmann B, Henriques JAP. Possible repair action of VitaminC on DNA damage induced by methyl methane sulfonate, cyclophosphamide, FeSO4 andCuSO4 in mouse blood cells in vivo. Mutat Res 2005; 583: 75–84.

Buege JA, Aust SD. Microsomal Lipid Peroxidation. Methods Enzymol 1978; 52:302-10.

Erel 0. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry 2004; 37: 277– 85.

Erel 0. A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry 2005; 38:1103–11.

Jansen EHJM, Ruskovska T. Comparative Analysis of Serum (Anti)oxidative Status Parаmeters in Healthy Persons. Int J Mol Sci 2013; 14:6106-15.

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without the use of preparative centrifuge. Clinical Chemistry 1972; 18: 499-502.

Tiffin N, Adeyemo A, Okpechi I. A diverse array of genetic factors contribute to the pathogenesis of Systemic Lupus Erythematosus. Orphanet J Rare Dis 2012; 8: 1-8.

Bijl M, Reefman E, Horst G, Limburg P C, Kallenberg CG. Reduced uptake of apoptotic cells by macrophages in systemic lupus erythematosus: correlates with decreased serum levels of complement. Ann Rheum Dis 2006; 65:57–63.

Munoz LE, van Bavel C, Franz S, Berden J, Herrmann M, van der Vlag J. Apoptosis in the pathogenesis of systemic lupus erythematosus. Lupus 2008; 17:371–75.

Sung CC, Hsu YS, Chen CC, Lin YF, Wu CC. Oxidative Stress and Nucleic Acid Oxidation in Patients with Chronic Kidney Disease. Oxid Med Cell Longev 2013; 1-15.

Stoyanova E, Sandoval SB, Zúñiga LA, El-Yamani N, Coll E, Pastor S, Reyes J, Andrés E, Ballarin J, Xamena N, Marcos R. Oxidative DNA damage in chronic renal failure patients. NDT 2010; 25:879-85.

Manzi S, Agarwal S, Elliott JR. Atherosclerosis risk factors in systemic lupus erythematosus. Curr Rheumatol Rep 2009; 11:241–7.




DOI: http://dx.doi.org/10.7439/ijbr.v6i7.2213

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