GC-MS analysis of bio-active compounds in the methanolic extract of Ghee Residue

P. Praveen Kumar, V. Ranjith, R. Subash, Gowri Shankar B A


Ghee residue is a disuse by-product of ghee manufacturing industry and it is a rich potential source of natural antioxidants. It is used as the ingredient for the preparation of chocolates, cakes and cookies in many industries because of the presence of high protein content in it. The aim of this study is to identify and characterize the vital bioactive compounds from the methanolic extract of ghee residue by Gas chromatography and Mass spectroscopy (GC-MS). The GC-MS analysis of the methanolic extract revealed the presence of 30 bioactive compounds. Among the 30 compounds, the most predominant compounds are fatty acids like hexadecanoic acid, tetradecanoic acid, dodecanoic acid, octadecanoic acid and an amino acid N-glycyl L-threonine. This study forms a basis for the biological characterization and importance of the compounds which could be exploited for future development of drugs.


GC-MS analysis; bioactive compounds; ghee residue; antioxidant.

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Shukla DJ, Vyas HA, Vyas MK, Ashok BK, Ravishankar B. A comparative study on chronic administration of Go Ghrita (cow ghee) and Avika Ghrita (ewe ghee) in albino rats. Ayu. 2012;33(3):435–40.

Patwardhan B, Datta HS, Mitra SK. Wound healing activity of topical application forms based on ayurveda. Evidence-based Complement Altern Med. 2011;2011.

Mortensen BK. Anhydrous Milk Fat/Butter Oil and Ghee. Ref Modul Food Sci. 2016;515–21.

Rasheeda Khanam RGP and SA. Evaluation of Total Phenolic Content in Ghee Residue: Contribution to Higher Laccase Production. Microbiol J. 2013;3:12–20.

Arumugam MP, Vedhanayagam K, Doraisamy KA, Narahari D. Chemical composition and nutritive value of ghee residue for chickens. Anim Feed Sci Technol. 1989;26(1–2):119–28.

Borawake KA, Bhosale DN. Utilization of ghee residue in preparation of nankatai type cookies and sponge cakes. Indian J Dairy Sci. 1996;49(2):114–9.

Kelly DR, Harrison SJ, Jones S, Wadding A, Mazomenos BE, Raptopoulos D. The synthesis and biological activity of analogs of 1,7-dioxaspiro[5.5]undecane. The pheromone of the olive fly (Bactrocera oleae). BCPC Symp Proc. 1995;63:387–94.

Obniska J. Synthesis and anticonvulsant properties of a series of N-substituted 2-aza-spiro[4.5]decane-1,3-diones and 8-phenyl-2-aza-spiro[4.5]decane-1,3-diones. Acta Pol Pharm - Drug Res. 2004;61(6):467–72.

Iwasawa N, Hayakawa S, Funahashi M, Isobe K, Narasaka K. Generation of .BETA.-Carbonyl Radicals from Cyclopropanol Derivatives by the Oxidation with Manganese(III) 2-Pyridinecarboxylate and Their Reactions with Electron-Rich and -Deficient Olefins. Bull Chem Soc Jpn. 1993;66(3):819–27.

Ryabchuk P, Edwards A, Gerasimchuk N, Rubina M, Rubin M. Dual control of the selectivity in the formal nucleophilic substitution of bromocyclopropanes en route to densely functionalized, chirally rich cyclopropyl derivatives. Org Lett. 2013;15(23):6010–3.

Baig U, Gondal MA, Alam MF, Wani WA, Younus H. Pharmacological evaluation of poly(3-methylthiophene) and its titanium(IV)phosphate nanocomposite: DNA interaction, molecular docking, and cytotoxic activity. J Photochem Photobiol B Biol. 2016;164:244–55.

Ryu JM, Han HJ. L-threonine regulates G1/S phase transition of mouse embryonic stem cells via PI3K/Akt, MAPKs, and mTORC pathways. J Biol Chem. 2011;286(27):23667–78.

Kang KS, Yamabe N, Kim HY, Yokozawa T. Role of maltol in advanced glycation end products and free radicals: in-vitro and in-vivo studies. J Pharm Pharmacol. 2008;60(4):445–52.

Kim Y-B, Oh SH, Sok D-E, Kim MR. Neuroprotective effect of maltol against oxidative stress in brain of mice challenged with kainic acid. Nutr Neurosci. 2004;7(1):33–9.

Hong S, Iizuka Y, Lee T, Kim CY, Seong GJ. Neuroprotective and neurite outgrowth effects of maltol on retinal ganglion cells under oxidative stress. Mol Vis. 2014;20:1456–62.

Cechovska L, Cejpek K, Konecny M, Velisek J. On the role of 2,3-dihydro-3,5-dihydroxy-6-methyl-(4H)-pyran-4-one in antioxidant capacity of prunes. Eur Food Res Technol. 2011;233(3):367–76.

Roth BD, Blankley CJ, Chucholowski AW, Ferguson E, Hoefle ML, Ortwine DF, et al. Inhibitors of cholesterol biosynthesis. 3. Tetrahydro-4-hydroxy-6-[2-(1H-pyrrol-1-yl)ethyl]-2H-pyran 2-one inhibitors of HMG-CoA reductase. 2. Effects of introducing substituents at positions three and four of the pyrrole nucleus. J Med Chem. 1991;34(1):357–66.

Lee M, Jin Y, Kim DH. 2-Benzyl-2-methylsuccinic acid as inhibitor for carboxypeptidase A. synthesis and evaluation. Bioorganic Med Chem. 1999;7(8):1755–60.

Rosatella A a., Simeonov SP, Frade RFM, Afonso C a. M. 5-Hydroxymethylfurfural (HMF) as a building block platform: Biological properties, synthesis and synthetic applications. Green Chem. 2011;13(4):754.

Alves RC, Costa ASG, Jerez M, Casal S, Sineiro J, Núñez MJ, et al. Antiradical activity, phenolics profile, and hydroxymethylfurfural in espresso coffee: Influence of technological factors. J Agric Food Chem. 2010;58(23):12221–9.

Michail K, Matzi V, Maier A, Herwig R, Greilberger J, Juan H, et al. Hydroxymethylfurfural: An enemy or a friendly xenobiotic. A bioanalytical approach. Anal Bioanal Chem. 2007;387(8):2801–14.

Kasurinen J. A novel fluorescent fatty acid, 5-methyl-BDY-3-dodecanoic acid, is a potential probe in lipid transport studies by incorporating selectively to lipid classes of BHK cells. Biochem Biophys Res Commun. 1992;187(3):1594–601.

Schwantes U, Dautel H, Jung G. Prevention of infectious tick-borne diseases in humans: Comparative studies of the repellency of different dodecanoic acid-formulations against Ixodes ricinus ticks (Acari: Ixodidae). Parasit Vectors. 2008;1(1):8.

Sivakumar R, Jebanesan A, Govindarajan M, Rajasekar P. Oviposition attractancy of dodecanoic, hexadecanoic and tetradecanoic acids against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Eur Rev Med Pharmacol Sci. 2011;15(10):1172–5.

Krug HF, Oberle C, Matzke A, Massing U. The antiproliferative alkylphospholipid S-1-O-phosphocholine-2-N-acetyl-octadecane induces apoptosis in leukemia cell lines. Ann N Y Acad Sci. 2003;1010:335–8.

Ribeiro SB, Pagnocca FC, Victor SR, Bueno OC, Hebling MJ, Bacci Jr. M, et al. Activity of sesame leaf extracts against the symbiotic fungus of Atta sexdens L. An Soc Entomol Bras. 1998;27:421–42.

Gupta S, Pandeya SN, Kumar P, Tripathi L, Prashant P, Anurag, et al. Synthesis and analgesic activity of some new benzoyl derivatives. Asian J Chem. 2007;19(4):2891–5.

Gupta S, Pandeya SN, Kumar P, Murti K, Prashant P, Verma KK, et al. Synthesis and analgesic activity of 7-isopropyl-4-methyl-1-(substituted phenyl sulfonyl)-azepan-2-ones. Indian J Heterocycl Chem. 2006;16(2):193–4.

Adachi K, Yokoyama D, Tamai H, Sadai M, Oba K. Effect of the glyceride of pentadecanoic acid on energy metabolism in hair follicles. Int J Cosmet Sci. 1993;15(3):125–31.

Smedman AE, Gustafsson I-B, Berglund LG, Vessby BO. Pentadecanoic acid in serum as a marker for intake of milk fat: relations between intake of milk fat and metabolic risk factors. Am J Clin Nutr. 1999;69(1):22–9.

Okamoto M, Ibanez P. Final Report on the Safety Assessment of Oleic Acid, Laurie Acid, Palmitic Acid, Myristic Acid, and Stearic Acid. Int J Toxicol. 1987;6(3):321–401.

Cartron ML, England SR, Chiriac AI, Josten M, Turner R, Rauter Y, et al. Bactericidal activity of the human skin fatty acid cis-6-hexadecanoic acid on Staphylococcus aureus. Antimicrob Agents Chemother. 2014;58(7):3599–609.

Piantoni P, Lock a L, Allen MS. Palmitic acid increased yields of milk and milk fat and nutrient digestibility across production level of lactating cows. J Dairy Sci. 2013;96(11):7143–54.

Sohn HR, Baek KY, Hou CT, Kim HR. Antibacterial activity of 7,10-dihydroxy-8(E)-octadecenoic acid against food-borne pathogenic bacteria. Biocatal Agric Biotechnol. 2013;2(1):85–7.

Bano T, Kumar N, Dudhe R. Free radical scavenging properties of pyrimidine derivatives. Org Med Chem Lett. 2012;2(1):34.

Flynn PJ, Reece RJ. Activation of Transcription by Metabolic Intermediates of the Pyrimidine Biosynthetic Pathway. Mol Cell Biol. 1999;19(1):882–8.

Ramesh B, Bhalgat CM. Novel dihydropyrimidines and its pyrazole derivatives: Synthesis and pharmacological screening. Eur J Med Chem. 2011;46(5):1882–91.

Karabay-Yavasoglu NU, Sukatar A, Ozdemir G, Horzum Z. Antimicrobial activity of volatile components and various extracts of the red alga Jania rubens. Phyther Res. 2007;21(2):153–6.

Nalawade T, Sogi SP, Bhat K, Bhat K, Sogi SHP, Sogi SHP, et al. Bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400, and polyethylene glycol 1000 against selected microorganisms. J Int Soc Prev Community Dent. 2015;5(2):114.

Vohora SB, Shamsi MA, Khan MSY. Antipyretic and analgesic studies on the diacetate of a new triterpenic acid isolated from Corchorus depressus L. J Ethnopharmacol. 1981;4(2):223–8.

Holesova S, Valaskova M, Pazdziora E, Matejova K. Preparation of novel organovermiculites with antibacterial activity using chlorhexidine diacetate. J Colloid Interface Sci. 2010;342(2):593–7.

Drummy LF, Kübel C, Martin DC. Molecular vacancies in herringbone crystals. Philos Mag. 2004;84(19):1955–76.

Yang HO, Suh DY, Han BH. Isolation and characterization of platelet-activating factor receptor binding antagonists from Biota orientalis. Planta Med. 1995;61(1):37–40.

Mo H, Trogisch S, Taub H, Ehrlich SN, Volkmann UG, Hansen FY, et al. Studies of the structure and growth mode of dotriacontane films by synchrotron x-ray scattering and molecular dynamics simulations. J Physics-Condensed Matter. 2004;16(29):S2905--S2910.

Chauhan A, Sharma PK, Kaushik N. Pyrazole: A versatile moiety. Vol. 3, International Journal of ChemTech Research. 2011. p. 11–7.

Khan MF, Alam MM, Verma G, Akhtar W, Akhter M, Shaquiquzzaman M. The therapeutic voyage of pyrazole and its analogs: A review. Vol. 120, European Journal of Medicinal Chemistry. 2016. p. 170–201.

Keter FK, Darkwa J. Perspective: The potential of pyrazole-based compounds in medicine. Vol. 25, BioMetals. 2012. p. 9–21.

Donio MBS, Ronica SFA, Viji VT, Velmurugan S, Jenifer JA, Michaelbabu M, et al. Isolation and characterization of halophilic Bacillus sp. BS3 able to produce pharmacologically important biosurfactants. Asian Pac J Trop Med. 2013;6(11):876–83.

Cole N, Hume EBH, Jalbert I, Kumar Vijay A, Krishnan R, Willcox MDP. Effects of topical administration of 12-methyl tetradecanoic acid (12-MTA) on the development of corneal angiogenesis. Angiogenesis. 2007;10(1):47–54.

Othman Razi A, Abdullah N, Ahmad S, Ismail Safinar I, Zakaria Pauzi M. Elucidation of in-vitro anti-inflammatory bioactive compounds isolated from Jatropha curcas L. plant root. BMC Complement Altern Med. 2015;15(1):1–10.

Tamokou J De, Simo Mpetga D, Keilah Lunga P, Tene M, Tane P, Kuiate J. Antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds from stem bark of Albizia adianthifolia (Mimosoideae). BMC Complement Altern Med. 2012;12(1):99.

Maher P, Akaishi T, Schubert D, Abe K. A pyrazole derivative of curcumin enhances memory. Neurobiol Aging. 2010;31(4):706–9.

DOI: http://dx.doi.org/10.7439/ijpc.v7i3.4018

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