Screening of the Ethnobotanicals against MDA-MB-231 and MCF-7 breast cancer cell lines

Mohammed Shafi Sofi, M K Sateesh, Mohsin Bashir

Abstract


The present study aims to investigate the therapeutic properties of a library of the medicinal plants for their potential anticancer activity against breast cancer cells. The viability of MDA-MB-231 and MCF-7 breast cancer cells was determined by MTT and trypan blue exclusion assays. The cellular cytotoxicity and the levels of cytosolic enzyme Lactate Dehydrogenase (LDH) were measured by assessing ┬Ámoles of NADH/well/min. Additionally DNA laddering and FACS analysis was done to further investigate the nature of cell death in these cells. Out of the tested plants, only Abrus precatorius L. extract showed a very significant cytotoxic activity on both cell lines. Trypan-blue assay showed the cell viability was significantly decreased in a dose dependant manner in both cell lines after 48 h of treatment. On the other hand, LDH activities in the cultured media were significantly elevated in both cell lines in a dose dependant manner as compared to the control. A prominent DNA laddering pattern in agarose gel was seen, which is an important hallmark of apoptosis. FACS analysis confirmed the apoptosis by the appearance of Sub G0/G1 peak in treated cells as compared to the control. This analysis will facilitate the use of traditional medicine in cancer treatment.


Keywords


Ethanobotany, Abrus precatorius L., Breast cancer, Cytotoxicity

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DOI: http://dx.doi.org/10.7439/ijpp.v4i6.123

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