Purification and characterization of endophytic fungal strains from four different high value medicinal plants of Kashmir valley

Refaz Ahmad Dar, Shabir a rather, Saleem mushtaq, Parvaiz Hassan Qazi


This study was designed to isolate, purify and characterize endophytic fungi from four different high value medicinal plants of Kashmir valley as a possible source of bioactive secondary metabolites. A total of 14 morphologically different endophytic strains were isolated from the plants exploited for endophytes. All isolates were identified based on colony morphology, examination of spores and light microscopes. The endophytic fungi were successfully isolated from the plants using the surface disinfection method. The optimized parameters for the optimum growth of the endophytic fungi were obtained as temperature of 28C, potato dextrose broth medium and rpm at 180-200.


Endophyte, secondary metabolite, fungi

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Arnold AE, Maynard Z, Gilbert GS, Coley PD, Kursar TA. Are tropical fungal endophytes hyperdiverse? Ecology Letters, 3(4), 2000, 267274.

Carroll MC. The complement system in regulation of adaptive immunity. Nature Immunology, 5(10), 2004, 981-986.

Dreyfuss MM and Chapela IH. Potential of fungi in the discovery of novel, low-molecular weight pharmaceuticals. In: The discovery of Natural Products with Therapeutic Potential (ed. V.P. Gullo). Butterworth-Heinemann, London, UK, (1994), 49-80.

Frhlich J and Hyde K D. Biodiversity of palm fungi in the tropics: are global fungal diversity estimates realistic? Biodiversity Conservation, 8, 1999, 977-1004.

Gunatilaka AAL. Natural products from plant-associated microorganisms: distribution, structural diversity, bioactivity, and implications of their occurrence. Journal of Natural Products, 69, 2006, 509-506.

Kumaran A, and Karunakaran RJ. Antioxidant activity of Cassia auriculata flowers. Fitoterapia, 78, 2007, 46 47.

lumyong S, lumyong P, hyde KD. Endophytes, in Thai Fungal Diversity (Ed. E. B. G. Jones, M. Tanticharoen and K. D. Hyde), National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand, 2004, 197-205.

Owen NL, Hundley N. Endophytes--the chemical synthesizers inside plants. Science progress, 87(2), 2004, 79-99.

Rodrigues K. F. The foliar fungal endophytes of the Amazonian palm Euterpe oleracea. Mycologia, 86, 1994, 376-85.

Strobel G, and Daisy B. Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews, 67, 2003, 491502.

Tan RZ and Zou WX. Endophytes: a rich source of functional metabolites. Natural product Reports, 18, 2001, 448-459.

Vainio EJ, Korhonen K, Hantula J. Genetic variation in Phlebiopsis gigantean as detected with random amplified microsatellite (RAMS) markers. Mycological Research, 2, 1998, 187-192.

Verma V, Rehman S, Shawl AS, , Kour A, Athar M, Andrabi R, Sultan P, Qazi GN. An endophytic Neurospora sp. from Nothapodytes foetida producing camptothecin. Applied Biochemistry and Microbiology, 44(2), 2008, 203-9.

Wilson D. and G. C. Carroll. Infection studies of Discula quercina, an endophyte of Quercus garryana. Mycologia, 86, 1994, 635-647.

DOI: https://doi.org/10.7439/ijpp.v5i1.1596


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