Pharmacognostical Standardization, Phytochemical Analysis and In-vitro Antioxidant Activity of rhizome of Polygonum alpinum All.

Mir P. A., Naqash A, Shah M. Y., Bhat Z. A., Bader G. N.

Abstract


The present study was designed to evaluate the pharmacognostic characters, physicochemical parameters and in-vitro antioxidant activity of rhizome of Polygonum alpinum. The powder microscopy revealed the presence of epidermis, hypodermis with calcium oxalate crystals, non lignified vascular bundle, calcium oxalate crystals, cork in surface view and fragments of bordered pitted vessels. Phytochemical screening of various extracts revealed the presence of carbohydrates, cardiac glycosides, coumarin, steroids, terpenoids, flavonoids, saponins and tannins. Physiochemical analysis showed 2.325% of total ash, 0.525% of water soluble ash, 0.650% of acid insoluble ash and 3.625% of sulphated ash w/w. Other parameters like extractive value, foreign matter, moisture content, swelling index, foaming index, pH of different solvents, total tannin content, total fat content and fluorescence analysis were also determined. The total phenolics in alcoholic and aqueous extract was found to be 41.06 and 23.30 mg/g GAE respectively while that of flavonoid content in alcoholic and aqueous extract was 182.13 and 123.71 mg/g RE respectively. The percentage inhibition of DPPH by alcoholic and aqueous extract at a concentration of 100 μg/mL was found to be 96.50% and 49.44% respectively. The reducing power of alcoholic and aqueous extract at 100 μg/mL was found to be 0.295 and 0.175 respectively and increased to 0.765 and 0.596 respectively at 500 μg/mL. The results indicate that alcoholic extract has potent antioxidant activity as compared to the aqueous extract.

Keywords


Polygonum alpinum; DPPH; reducing power; pharmacognostic characters

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References


. Kaul MK, Katiyar SK & Sharma PK, Nutritive value and medicinal beliefs of some novel wild plants of Gurez valley in Kashmir, Arogya - J HlthSci, 16 (1990) 31-134.

. Chittendon, F. 1956. RHS dictionary of plants plus supplement. Oxford University Press, U.K.

. Hedrick. U. P. Sturtevant's Edible Plants of the World. Page 511

. Singh, Dr. G. and Kachroo. (1976). Prof. Dr. P. Forest Flora of Srinagar. Bishen Singh Mahendra Pal Singh.

. CK Kokate; AP Purohit; SB Gokhale. Pharmacogognosy, 35th ed., Nirali Prakashan, Pune 2006; 98-114.

. Mehlhorn H. Nature helps: How plants and other organisms contribute to solve health problems. Germany: Springer; 2011 p. 12.

. Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Int Pharm Sci 2011; 1(1): 98- 106.

. Menpara D, Chanda S. Phytochemical and pharmacognostic evaluation of leaves of Pongamia pinnata L. (Fabaceae). Phcog Commun 2014; 4(2): 3-7.

. Surendra Kumar Sharma and Ajay Pal Singh. Morphological, microscopical and physico-chemical investigations on the rhizomes of Cyperus rotundus Linn. Research Journal of Pharmaceutical, Biological and Chemical Sciences 2011; 2 (3); 798.

. Mukherjee PK. Quality Control of Herbal Drug, 1st Edition, Business Horizons Pharmaceutical Publishers, New Delhi 2010: 184-191.

. Mukherjee PK. Quality control herbal drugs: An Approach to Evaluation of Botanicals,4 th edition, Business Horizons Pharmaceutical Publishers, New Delhi, 2008: 183-195.

. Quality control methods for herbal materials. WHO Library Cataloguing in Publication data. pp. 2011; 9: 45-49.

. Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I. Antioxidant principles from Bauhinia terapotensis. J Nat Prod. 2001; 64:892-895.

. M. Akbar Dar , Mubashir H. Masoodi , Adil F. Walia, Mudasir A. Mira, Nida S. Shapoob. Antioxidant potential of methanol Root extract of Mentha arvensis L. from Kashmir Region. Journal of Applied Pharmaceutical Science 2014; 4 (03): 050-057.

. Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jap. J. Nutr 1986; 44: 307- 315.

. Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M. Free radical scavenging properties of wheat extracts. J. Agric. Food Chem. 2002; 50: 1619–1624.

. C. Chang, M. Yang and H. Wen. Cheru J. Estimation of total flavonoids content in propolis by two complementary colormetric methods. J. Food Drug Anala 2002; 10: 178-82.

. Tepe B, Daferera D, Sokmen A, Sokmen M, Polissiou M. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food Chemistry 2005; 90: 333–340.

. Lee SE, Hwang HJ, Ha JS, Jeong HS, Kim JH. Screening of medicinal plant extracts for antioxidant activity. Life Sci 2003; 73: 167-179.

. Shajeesh T, Arunachalam K, Parimelazhagan T. Antioxidant and antipyretic studies on Pathos scandens L. Asian Pac J Trop Med, 2011; 4: 889-899.

. Poongothai K, Ponmurugan P, Syed Zameer AK, Senthil KB, Sheriff SA. Antihyperglycemic and antioxidant effects of Solanum xanthocarpum leaves (field grown & in vitro raised) extracts on alloxan induced diabetic rats. Asian Pac J Trop Med 2011; 4: 778-785.

. Umamaheswari M, Chatterjee TK. In vitro antioxidant activities of the functions of Coccinia grandis L extract. Afr J. Trad. Corrpl aler. Med., 2008; 5: 61-73.

. Wagh S.S., Jain S.K., Patil A.V., Vadnere G.P. In vitro free radical scavenging and antioxidant activity of Cicer arietinum L. (Fabaceae). Int. J. PharmTech. Res. 2012; 4 (1): 343–350

. Chanda S. Importance of pharmacognostic study of medicinal plants: an overview. J Phcog Phytochem 2014; 2(5): 69-73.

. Singh S, Manchawal L, Chauhan MG. Pharmacognostic study of male leaves of Trichosanthes dioica Roxb. with special emphasis on microscopic technique. J Pharmacogn Phytother 2010; 2 (5):71–75.

. Jaitak, V.; Sharma, K.; Kalia, K.; Kumar, N.; HP, S.; Kaul, V.; Singh, B. Antioxidant activity of Potentilla fulgens: An alpine plant of western Himalaya. J. Food Compost. Anal. 2010; 23: 142-147.

. Othman, A.; Ismail, A.; Abdul Ghani, N.; Adenan, I. Antioxidant capacity and phenolic content of cocoa beans. Food Chem. 2007; 100: 1523-1530.

. Scalzo, J.; Politi, A.; Pellegrini, N.; Mezzetti, B.; Battino, M. Plant genotype affects total antioxidant capacity and phenolic contents in fruit. Nutrition 2005; 21: 207-213.

. Giorgi, M.; Capocasa, F.; Scalzo, J.; Murri, G.; Battino, M.; Mezzetti, B. The rootstock effects on plant adaptability, production, fruit quality, and nutrition in the peach (cv. 'Suncrest'). Sci. hortic. 2005; 107: 36-42.

. Gorinstein, S.; Vargas, O.; Jaramillo, N.; Salas, I.; Ayala, A.; Arancibia-Avila, P.; Toledo, F.; Katrich, E.; Trakhtenberg, S. The total polyphenols and the antioxidant potentials of some selected cereals and pseudocereals. Eur. Food Res. Technol. 2007; 225: 321-328.

. Ling, L.T.; Radhakrishnan, A.K.; Subramaniam, T.; Cheng, H.M.; Palanisamy, U.D. Assessment of Antioxidant Capacity and Cytotoxicity of Selected Malaysian Plants. Molecules 2010; 15: 2139-2151.

. Moure, A.; Cruz, J.; Franco, D.; Domínguez, J.; Sineiro, J.; Domínguez, H.; José Núñez, M.; Parajó, J. Natural antioxidants from residual sources. Food Chem. 2001; 72, 145-171.

. Turkoglu A, Duru MF, Mercan N, Kivrak I, Gezer K. Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.)Murill. Food Chem 2007; 101: 267-273.

. Jose S, Radhamany PM. Identification and determination of antioxidant constituents of bioluminescent mushroom. Asian Pac J Trop Biomed 2012; 2:S386-S391.




DOI: http://dx.doi.org/10.7439/ijpp.v7i5.4367

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