Evaluation of anti tumour activity of ethanolic extract of beet root (Beta vulgaris) against EAC mouse tumor model

P. Sravan Kumar, Asish Bhaumik, Domandula Babu Rao, Monica Chopra

Abstract


Introduction: EAC is referred to as undifferentiated carcinoma, and is originally hyper-diploid. The permeability to water is highest at the initiation of S and progressively decreases to its lowest value just after mitosis. Activation heats for water permeability vary during the cell cycle, ranging from 9–14 k. ca/mole.

Methodology: The extraction of phytoconstituents was carried out by reflux condensation and preliminary phytochemical screening was done by various qualitative confirmatory tests. The in vivo anti tumour activity of ethanolic extract of beet root (EEBT) was carried out on EAC mouse tumour models.

Results: All five animals were in both 5-FU as well as EEBT group. So mortality was less in both 5-FU and EEBT group. The Hb and RBC count were lower in tumour control group. The WBC counts were significantly increased in tumour control. 5-FU and EEBT mg/kg group decreased the WBC counts approximately near to normal range. The neutrophils were increased and lymphocytes were decreased significantly in tumour control group. The platelet count was also significantly increased in tumour control and EEBT (200 mg/kg) group compared nearly to normal group. EEBT and 5-FU significantly increased the PILS. While 5-FU increased the life span of EAC 90.90% and EEBT increased the life span of EAC 86.36%.

Conclusion: From the present experimental data here we concluded that the EEBT possessed potential anti tumour activity against EAC mouse tumour model which was proved by the assessment of haematological parameters as well as by Percent increase of lifespan (% ILS). The Percent increase of lifespan ( PILS) of both standard group as well as  EEBT treated group was found to be 90.90% and 86.36%.


Keywords


EAC, carcinoma, anti tumour activity, PILS

Full Text:

PDF

References


"A synopsis of Chenopodiaceae subfam. Betoideae and notes on the taxonomy of Beta; USDA PLANTS" (PDF) Willdenowia.

"Spinach, Beet and Swiss Chard - Notes - HORT410 - Vegetable Crops - Department of Horticulture and Landscape Architecture - Purdue University". Hort.purdue.edu. Retrieved 2010-09-12.

http://www.avrdc.org/pdf/seeds/beet.pdf

"Sugar beet". Agronomy.unl.edu. Archived from the original on March 29, 2007. Retrieved 2010-09-12.

"Integrative Biology 335: Systematics of Plants". Life.illinois.edu. Retrieved 2010-09-12.

Zeldes, Leah A. (2011-08-03). "Eat this! Fresh beets, nature’s jewels for the table". Dining Chicago. Chicago's Restaurant & Entertainment Guide, Inc. Retrieved 2012-08-03.

Hanelt, Peter; Büttner, R.; Mansfeld, Rudolf; Kilian, Ruth (2001). Mansfeld's Encyclopedia of 8. Agricultural and Horticultural Crops. Springer. pp. 235–241. ISBN 3-540-41017-1.

Grubben, G.J.H. & Denton, O.A. (2004) Plant Resources of Tropical Africa 2. Vegetables.

PROTA Foundation, Wageningen; Backhuys, Leiden; CTA, Wageningen.

Keritot 6a; Horiyot 12a; Rabbenu Nissim at the end of Rosh Hashana, citing the custom of Rav Hai Gaon; Abudraham; Shulchan Aruch OC 583:1

Bansal Raj. K., Laboratory manual of organic chemistry, 5th revised edition, PP- 238-239.

Dandiya P.C, Sharma P.K., Bio-chemistry and clinical pathology, second edition, PP- 17-18, 24, 47-48.

Rao G. Devala, A Manual of Practical Biochemistry, pp 17.

Jaswant Kaur, PV Chemistry of Natural Products, 2010 edition, PP-113-114, 116, 344-346, 381.

“OECD guidelines – 423” for testing of chemicals, 2001, pp 1-14.

Evaluation of in vivo antitumor activity of cleistanthin B in Swiss albino mice. Journal of Traditional and Complementary Medicine, 2016; 6(4): 383–388.

Raihan M.O., Tareq S.M., Brishti A., Alam M.K., Haque A., Ali M.S. Evaluation of antitumor activity of Leea indica (Burm.f.) Merr. extract against Ehrlich ascites carcinoma (EAC) bearing




DOI: http://dx.doi.org/10.7439/ijpr.v7i3.3943

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 International Journal of Pharmacological Research

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.