Cross-cytotoxicity study of antimalarial drugs and traditional medicinal plants on Renal Cells

Thérèse Daubrey-Potey, Serges Aoussi, Valéry Adjogoua, Gisèle Kouakou-sirensy, Mamadou Kamagaté, Mireille Dosso, Henri Die-Kacou

Abstract


Objective:  The association of Artemisinin based combination therapy with herbal medicine seems to be a factor favouring the occurrence of serious adverse effects, hepatitis disorders and acute renal failure in particular. The aim of this study was to evaluate the cross- toxicity of these associations on renal cells.
Methods: African green monkey kidney cells (VERO) were incubated with anti-malarial drugs and plant preparations. The products used were: Artesunate 100mg / amodiaquine 270mg (ASAQ) and artemether 80mg / lumefantrine 480mg (AL) , and Sida acuta and Enantia polycarpa at a concentration of 10 μg / ml. After 5 days of incubation, a cell count was performed using a haemocytometer.
Results: ASAQ resulted in significant cell destruction compared to the control between Day2 (p <0.05) and Day5 (p <0.01) with a peak at day 3 (p <0.001). There was also a significant difference between Sida acuta and control on Day4 (p <0.05) and Day5 (p <0.001). Cell mortality was> 30% in the tubes treated with ASAQ and Sida acuta. Finally, cell destruction was statistically significant in tubes treated with the combination of antimalarial drugs and traditional plants compared to the control tube from Day2 (p <0.001).
Conclusion:  The renal toxicity observed with ASAQ, Sida acuta and combination of antimalarial drugs and herbs, appears dose-dependent or cumulative. The mechanism of this renal toxicity should be better analysed in further studies.

Keywords


Cytotoxicity, Artemisinine based Combination Therapy, Traditherapy, Malvaceae, Annonaceae

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References


Henry MC, Rogier C, Nzeyimana I, Assi SB, Dossou-Yovo J, Audibert M, et al. Inland valley rice production systems and malaria infection and disease in the savannah of Cote d’Ivoire. Trop Med Int Health. 2003;8:449-458.

WHO . Guidelines for the treatment of malaria. Geneva: World Health Organization; 2006

Balayssac E, Die-Kacou H, Yavo JC, Kakou KA, Kamagate M, Daubret-Potey T, et al. Interest of specific report systeme of adverse drug reaction due to antimalarial drugs in Cote d’Ivoire. Drug Safety 2005; 28(10): 959

Die-Kacou H, Kamagate M, Daubrey Pt, Yavo JC, Bakayoko A, Balayssac E. Management of risk in a country in crisis: hepatonephritis cases related to artemisinin based combination therapeutic use. Fundam Clin Pharmacol2009; 23(supplement 1): 32-33

Die-Kacou H, Yavo JC, Daubrey PT, Gnionsahe DA, Ake A. Factors associated with serious and expected adverse drug reaction risk of artemisinin based Combination (ACT). Fundam Clin Pharmacol2010; 24(suppl 1): 77

Falodun A. Herbal medecine in Africa. Distribution, standardization and prospects. Research Journal of Phytochemistry2010 ;4:154-161.

Anosa G, Udegbunam R, Okoro J, Okoroafor O. In vivo antimalarial activities of Enantia polycarpa stem bark against Plasmodium berghei berghei in mice. J Ethnopharmacol2014 Apr 28; 153(2):531-4.

Karou D, Dicko MH, Sanon S, Simpore J, Traore AS. Antimalarial activity of Sida acuta Burm. f. (Malvaceae) and Pterocarpus erinaceus Poir. (Fabaceae). J Ethnopharmacol 2003 Dec;89(2-3):291-4.

Banzouzi JT, Prado R, Menan H, Valentin A, Roumestan C, Mallié M, et al. Studies on medicinal plants of Ivory Coast: investigation of Sida acuta for in vitro antiplasmodial activities and identification of an active constituent. Phytomedicine 2004;11(4):338-41.

Coulerie M. P. Étude phytochimique et pharmacologique de plantes de Nouvelle-Calédonie à potentialités anti-dengue [Thèse].Nouvelle-Calédonie : Université de la Nouvelle-Calédonie ; 2012

Almoradie J, Ortiz JT. Black water fever : a twelve year study. J Phil Asso 1993 ; 59: 15-9

Assa JL. Hémolyse intravasculaire due aux antipaludiques : contribution à l’analyse de la relation causale [Thèse]. Abidjan : Université Félix Houphouët Boigny; 2001 ( 3125).

Assi LE. Contribution à l’étude de la fièvre bilieuse hémoglobinurique à propos de 13 cas observés à la PISAM en 1993 [Thèse]. Abidjan:Université Félix Houphouët Boigny; 1994 (1447).

Kramo AD Hémolyse aiguë intravasculaire d’origine médicamenteuse au cours du traitement antipaludique : analyse et réflexions à partir de 25 nouvelles observations [Thèse]. Abidjan:Université Félix Houphouët Boigny; 2000 (2449).

Daubrey-Potey T, Die-Kakou H, Kamagate M, Vamy M, Balayssac E et Yavo JC.Fièvre bilieuse hémoglobinurique au cours du traitement antipaludique à Abidjan : à propos de 41 cas. Bull Soc Pathol Exo 2004 ; 97(5) : 325-8

Kamagate M, Diallo CO, Meless D, Daubrey PT, Kakou A, BalayssacE, et al. Hepatonephritis during the treatment of malaria by the therapeutic combinations from a pharmacovigilance database: what reality? Fundam Clin Pharmacol2010 ; 30 (Suppl. 1) ; 5–24

Soro L, Brouh Y, Amonkou A, Aye YD et al. Intoxication mortelle à l’Artésunate : à propos d’un cas chez un enfant. Rev Int Sc Med 2006 ; 8(2) :49-52

Gevard E, Aguemon A. Deux hépatites fulminantes survenues au cours d’un traitement curatif par l’association artésunate-amodiaquine. Med Mal Infect 2009 ; 39 :57-60

Konaté K,Bassolé I, Hilou A, Aworet-Samseny R, Souza A, Barro N, et al. Toxicity assessment and analgesic activity investigation of aqueous acetone extracts of Sida acuta Burn f . and Sida cordifolia L. (Malvaceae), medicinal plants of Burkina Faso. BMC Complement Altern Med 2012; 12: 120.




DOI: http://dx.doi.org/10.7439/ijpr.v7i9.4413

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