Ovulatory Effect of Aloe Barbadensis Mill in Mature New Zealand Rabbit Does

Revathy Saravanan, V. Bavani

Abstract


Background: Ovulatory failure in females being the cause in 20-30% of infertility, the inability to conceive naturally or to carry out a pregnancy to full term or to deliver a healthy baby after trying for 1 year. Availability of ovulation inducing medicines and assisted reproductive technologies does not answer enough solution leading to search for new avenues in alternative therapies. Ovulatory effects of the Aloe Vera Gel extract (AVG) from Aloe Barbadensis Mill have been demonstrated in Letrozole induced PCOD rat models. Hence this study to assess the ovulatory effect of AVG extract using increase in parabasal cells in vaginal smear as the indirect indicator using mature female rabbits is planned.

Methodology: With the approval of Institutional Research and Animal ethics committee six mature non pregnant rabbit does weighing 2-2.5 kg showing ovulatory failure in each group received saline as control or AVG extract only 150mg/kg orally for 28 days or followed by hCG 120 I.U I.V on 29th day or only hCG. Leishman stained vaginal smears taken on 0day, 1st week, 4th week and 6th week for first 3 groups and 48, 72 and 120 Hours after hCG for IVth group were examined for superficial and parabasal cells under 100x magnification. The data was analyzed with two way ANOVA and Bon-ferrani tests for statistical significance of p value <0.05.

Results: Statistically significant decrease in superficial cells percentage (p-<0.001) and proportionate increase in parabasal cells percentages (p-<0.001) were observed in does received aloe vera alone and also hCG on 4th & 6th week and 72 Hours after receiving only HCG.(P-value of < 0.01).

Conclusion: AVG of 150mg/kg /day for 28 days has shown ovulatory effect like HCG. Further studies supported by hormonal assays and HPE or USG for confirmation is suggested.


Keywords


infertility,ovulatory effect of Aloevera gel

Full Text:

PDF

References


. The National Family Health Survey (NFHS), International Institute of Population Science (IIPS), designated by Ministry of Health and Family Welfare (MOHFW) Mumbai, Government of India. 2007

. Princy Louis Palatty, Pratibha S. Kamble, Meera Shirke, Sanjay Kamble, Manohar Revankar & Vijaya M. Revankar A clinical round up of the female infertility Therapy amongst Indians. Journal of Clinical and Diagnostic Research. 2012; 6(7): 1343-1349.

. Vinay K. Gupta and Seema Malhotra Pharmacological attribute of Aloe vera: Revalidation through experimental and clinical studies. An International Quarterly Journal of Research in Ayurveda. 2012; 33(2): 193196.

. Laxmipriya Nampoothiri and Radha Maharjan, Padamnabhi S. Nagar Effect of Aloe Barbadensis Mill. Formulation on Letrozole induced polycystic ovarian syndrome rat model. J. Ayurveda Integr Med., 2010; 1(4): 273279.

. Vogler B K and Ernst E. Aloe Vera: a systematic review of its clinical Effectiveness. British Journal of General Practice, 1999; 49: 823-828.

. Jasem Estakhr and Nasim Javdan. Spermatogenic activity of aloe vera in Adult male rats. Pharmacologyonline, 2011, 2: 886-889.

. Bowen R. Classification of vaginal epithelial cells, 1998 http://arbl.cvmbs.colostate.edu/hbooks/pathphys/reprod/vc/cells.html.downloaded on December 2007.

. Tsiligianni Th., Saratsi A., Besenfelder U., Anastasiadis A., Vainas E., Saratsis Ph., Brem G, the use of cytological examination of vaginal smears in the selection of rabbits For super ovulation. Theriogenology, 2004; 61: 989995.

. Ola S. I. And Oyegbade M.O. The influence of different contact levels with maleon the vaginal cytology in rabbits under the tropical humid condition.9th World Rabbit Congress Verona Italy, 2008:417-421.

. Kunde M.M., Proud T. The ineffectiveness of vaginal smears in predicting the oestrous Cycle in the rabbit. Am. J. Physiol., 1929; 88: 446-452.

. Gupta S.K. Antifertility agents, Drug screening methods 2nd edition, Jaypee publications- 2009: 126-133.

. Ola S.I. and Oyegbade M.O. buck effect on rabbit oestrous: vulva colour, vaginal lumen cells and ovarian follicle populations. World Rabbit Sci. 2012; 20: 71 79.

. Gaware. V. M, Parjane. S. K, Merekar Abhijit N, Pattan S. R., Dighe N. S, Kuchekar B. S. Godge Rahul K. Female infertility and its treatment by alternative medicine: A review Journal of Chemical and Pharmaceutical Research, 2009; s1 (1): 148-162.

. Rizzi C., Chiericato G. M., Dalle Zotte A. reproductive and physiological Responses of rabbit Does under different nutritive levels before the first parturition 9th World Rabbit Congress June 10-13, 2008 Verona Italy downloaded on 28-11-2013.

. WHO monographs on selected medicinal plantsVolume-1World Health Organization Geneva 1999:38-40.

. Aloe vera gel, Summary report, Committee for veterinary medicinal products The european agency for the evaluation of medicinal products veterinary medicines evaluation unit. The European agency for the evaluation of medicinal products veterinary medicines evaluation unit. Downloaded on-26-11-2015.

. Inder Sehgal, Wallace D. Winters, Michael Scott, Andrew David, Glenn Gillis, Thaya Stoufflet, Anand Nair, and Konstantine Kousoulas. Toxicologic Assessment of a Commercial Decolorized Whole Leaf Aloe Vera Juice, Lily of the Desert Filtered Whole Leaf Juice with Aloesorb Hindawi Publishing Corporation. Journal of Toxicology, 2013; Article ID 802453: 1-12.

. Mehrdad Modaresi and Alireza Khodadadi. The Effects of Aloe Vera Extract on Reproductive Parameters in Mice. International Conference on Biological, Environment And Food Engineering (BEFE-2014) August 4-5, 2014 Bali (Indonesia)

. Ali Shahraki, Ali Shahraki Mojahed, Jalil Afshar-oli. The effects of Hydroalcoholic Extract of Aloe vera gel on spermatogenesis of adult male rats. International Journal of Biosciences 2014; 5 (7): 158-165.




DOI: https://doi.org/10.7439/ijpr.v7i12.4469



Copyright (c) 2017 International Journal of Pharmacological Research

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.