Simultaneous estimation of atenolol and chlorthalidone in combine tablet dosage form by absorption ratio method using UV-Vis spectrophotometry
Keywords: ATN, CTN, UV- VIS, Spectrophotometry, Assay method, ARM
AbstractA simple, precise, reproducible, accurate, economical & rapid UV-VIS Spectrophotometric method have been developed and validated for the simultaneous estimation of ATN and CTN in tablet dosage form. This paper describes the absorption ratio method as a quantification parameter. The absorption ratio method (ARM) involves measurement of absorbance of sample solution is measured at 240.0 nm (Isobestic Point) and 251.0 nm (? max of CTN) and based on E 1% 1cm values at these wavelengths two set of equations were framed. The developed method obeys the beers law in the concentration range of 40-80g/mL for ATN and 10-50 g/mL for CTN. The recovery studies shows %RSD for ATN 0.21 and for CTN 1.34 by ARM method. The results of analysis have been validated statistically for accuracy, precision, repeatability, specificity and ruggedness. The method was successfully applied to the determination of these drugs in pharmaceutical dosage form.
Download data is not yet available.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).