Estimation of Ofloxacin in Bulk and Formulation by Second Order DerivativeUV-Spectrophotometric Methods

  • Shivaji Shinde Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra
  • Santosh Jadhav Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra
  • Rekha Kharat Department of Pharmaceutics, S ahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra
  • Afaque Ansari Department of Pharmaceutics, D.S.T.S Mandals College of pharmacy, Solapur, Maharashtra,
  • Ashpak Tamboli Department of Pharmaceutical chemistry, Sahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra
Keywords: Ofloxacin, Second order Derivative, Area under Curve (AUC).

Abstract

Simple, fast and reliable spectrophotometric methods were developed for determination of Ofloxacin in bulk and pharmaceutical dosage forms. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 295-301nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ofloxacin using 2-10μg/ml (r²=0.9947) for second order Derivative Area under Curve spectrophotometric method. The proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ofloxacin in pharmaceutical formulations.

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Published
2015-06-30
How to Cite
Shinde, S., Jadhav, S., Kharat, R., Ansari, A., & Tamboli, A. (2015). Estimation of Ofloxacin in Bulk and Formulation by Second Order DerivativeUV-Spectrophotometric Methods. International Journal of Advances in Scientific Research, 1(5), 217-220. https://doi.org/10.7439/ijasr.v1i5.1977
Section
Research Articles