HPTLC fingerprinting and antimicrobial activity of Azadirachta indica leaf extracts

  • Rahul S. Shivarkar Pharmacognosy Dept Sinhgad Institute of Pharmaceutical Sciences, Lonavala
  • Satish Bhise A202, Navakar Residency, Behind Bibvewadi Police Chowki, Pune 411037
  • V. Rama Mohan Gupta Pulla Reddy Institute of Pharmacy Near Dundigal Air Force Academy, Annaram (V), Jinnaram (M) Medak (Dt) 502313
Keywords: HPTLC finger printing, Antimicrobial, Azadirachta indica, Aqueous and Ethanolic Extract

Abstract

Azadirachta indica Juss which also Known as Neem plant used from ancient times for various human ailments as a traditional medicine. To appraise the scientific study for the use of Azadirachta indica, ethanolic extracts of the dried leaves of the plant were subjected to HPTLC finger printing and determination of anti-microbial activity of aqueous and ethanolic extract on five different species of bacteria and a fungus. The ethanolic extract of dried powdered leaves was evaluated by HPTLC finger printing using solvent system of Dichloromethane: ethanol (19:1) which exhibits the identity of different compounds present in the extracts. The antimicrobial activity of the concentrated extracts was evaluated by determination of the diameter of zone of inhibition against the microorganisms using agar well diffusion method. Both plant extracts had antimicrobial effects against the test organisms, however the aqueous extracts were found to show greater anti-microbial effect than ethanolic extract. Thus, the mean diameter zones of inhibition ranged from 0.04mm-50.00mm for aqueous extract and 0.40mm-20.00mm for ethanolic extract at the highest concentration of 50mg/ml. The finding of this study supports the use Azadirachta indica leaves and can be use in the treatment of various microbial infections as alternative systems of medicine.

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References

Chiranjini P., Kumar A., Pandey B., Sharma G., Mishra K., Herbal for Human Health and Disease: Prospectus and Challenges of 21st Century, Herbal Drugs, Jaypee, New Dehli, India, 3,2006, 1-5.

Kamboj V. P., Herbal Medicine, Current Science, 78, 2000, 35-39.

Natarajan V, Venugopal PV, Menon T. Effect of Azadiractita indica (Neem) on the growth pattern of dermatophytes. Taramani Chennai 2003 600:113.

Kirtikar and Basu, In Medicinal Plants, Vivek Vihar. New Delhi 1975 pp. 536.

Biswas K, Chattopadhyay I, Ranajit KB, Bandyopadhya U Biological activities and medical properties of neem (Azadirachta indica). Current Sciences. (2002). 82 (11): 1336 -1345.

Vaideki K, Chadhopadhyay Banarjee, RK, Bandhopadhyay, U Biological activities and medicinal properties of neem (Azadirachta indica), Reviewed article. Current Science, 82, 2007,1336-1345.

Chopra RN, Chopra IC, Handa KL, Kapur LD Indigenous Drugs of India, U.N. Dhur and sons, Kolkala. 1958, 595.

Mohammad Asif. A Review on Spermicidal Activities of Azadirachta. Journal of Pharmacognosy and Phytochemistry , 1( 5), 2013,61-68.

Rao DVK, Sing K, Chopra R, Chatra PC, Ramanujalu G. In vitro antibactericidal activity of neem oil. Indian Journal of Medicinal Research, 84, 1986: 314 – 316.

Subhash c. Mandal, sabita pal (dutta), tapan k. Maity,M.pal and b.p saha Pharmacognostic Profiles of Azadirachta indicaA.Juss.Leaves Vol. No XIX (3&4), April, 2000, 118,-120.

Taura DW, Oyeyi TI In–vitro antibacterial activity of the combinations of Ethanolic extracts of Annova comosus, Allium sativum and Aloe barbadensis in comparison with ciprofloxacin. Biological and Environmental Sciences Journal for the Tropics, 6(1), 2009,146 – 151.

Thormberry AI Susceptibility tests and diffusion test procedures. Journal of Clinical. Pathology 19: 1983,492-300.

Onoruvwe O., Antibacterial Evaluation and Phytochemical Screening of the Leaf of Dichrostachys Cinera. West African Journal of Biological Sciences. 1965 5(1): 65 – 70.

George WR. Native African Medicine. Department of Forestry Research, 1st edition Ibadan, 1980, 90-91.

Erique, Natural Remedies for Health and Well Being 5th Edition Orvit publishing Company, Mexico 1988, 359.
Published
2018-06-30
Section
Original Research Articles