A Effect of Vitamin E on glutathione reductase and reduced glutathione levels of male wistar albino rats infected with Trypanosomabruceibrucei
The research work was carried out to assess the antioxidant effect of Vitamin E on glutathione reductase and reduced glutathione levels of male wistar albino rats infected with Trypanosomabruceibrucei .24 albino rats were divided into six groups of four test animal per group namely: control, Trypanosome infected, Trypanosome + diaminazine treated Trypanosome + 0.1mg Vitamin E, Trypanosome + 0.5mg Vitamin E and Trypanosome + 1.0mg Vitamin E respectively. Glutathione reductase and reduced glutathione were determined in all albino rats using oxidation -Reduction process .The data was subjected to statistical analysis using SPSS version 20. The result of this study shows that there was no significant difference (p>0.05) in reduced glutathione (µ/mg) concentrations of 26.73+8.89,33.48±0.31, 29.30±0.50, 30.35±0.44, 29.90±0.26 and 28.45±2.25 for control, Trypanosome infected, Trypanosome + diaminazine treated ,Trypanosome + 0.1mg Vitamin E, Trypanosome + 0.5mg Vitamin E and Trypanosome + 1.0mg Vitamin E respectively. The results also showed that there was no significant difference (P>0.05) in glutathione reductase(µ/mg) concentrations of 18.42±1.03, 18.70±0.17, 17.22±1.06, 17.65±0.29, 17.65±0.90 and 17.42±1.01 for control, Trypanosome infected, Trypanosome + diaminazine treated ,Trypanosome + 0.1mg Vitamin E, Trypanosome + 0.5mg Vitamin E and Trypanosome + 1.0mg Vitamin E respectively. The study showed significant reduction (P<0.05) in glutathione reductase and reduced glutathione levels of Vitamin E treated albino rats compared with the Trypanosomabruceibrucei infected albino rats.The result of this study suggested that oral administration of Vitamin E reduced the glutathione reductase and reduced glutathione levels induced by Trypanosomabruceibrucei infection
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