Effect of vitamin E on hydrogen peroxide and nitrogen levels in male wistar albino rats infected with trypanosoma Brucei Brucei
This research work was conducted to assess the antioxidant effect of vitamin E on hydrogen peroxide and nitrogen oxide levels. 24 albino rats were divided randomly into six groups of four test animal per group. Group A served as control and were given normal rat chow and water. Group B served as trypanosome treated and were infected with 1×106 trypanosome. Group C were infected with 1×106 trypanosome and treated with the standard drug (diaminazene acetuate). Group D were infected with 1×106 trypanosome and treated with 0.1mg/kg body weight of vitamin E (low dose). Group E were infected with 1× 106 trypanosome and treated with 0.5mg/kg body weight of vitamin E (moderate dose). Group F were infected with 1×106 trypanosome and treated with 1.0mg/kg body weight (high dose) for 14 days. Hydrogen peroxide and nitric oxide were assayed by scavenging Assay methods. The result of this study shows that hydrogen peroxide levels showed a significant difference (p<0.05) when compared with value of 0.31±0.03 of the control except with groups treated with 0.5mg and 0.1mg of vitamin E which showed no significant difference (p>0.05) with values of 0.36±0.03 and 36±0.03 respectively. However, nitric oxide levels showed significant difference (p>0.05) when compared with value of 21.44±0.91 of control except group treated with 0.5mg of vitamin E which showed significant difference (p<0.05) with value of 17.09 ±0.00. The findings from this study therefore show that Vitamin E caused changes in Hydrogen peroxide and Nitric oxide levels based on the concentration of the vitamin E.
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