Association of Soluble α-Klotho and Klotho Polymorphisms (KL-VS and G-395A) with Essential Hypertension in Indian Population

  • Gauri Pathare Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Center
  • Sunila Raju Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Center
  • Vinod Shah Sir H.N. Reliance Foundation Hospital and Research Center
  • Manoj Mashru Sir H.N. Reliance Foundation Hospital and Research Center
  • Kavita Shalia Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Center
Keywords: Single nucleotide polymorphism, Klotho, anti-aging gene, cardiovascular disease

Abstract

Klotho, a novel anti-aging gene, is known to ameliorate aging phenotypes and extend lifespan. Recent studies have highlighted arterial aging as a risk factor for hypertension. Thus, Klotho appears to be a link between aging and cardiovascular diseases (CVD) including hypertension. KL-VS and G-395A polymorphisms of Klotho and its soluble levels have been associated with CVD incidence. We analyzed the prevalence of α-Klotho polymorphisms – KL-VS and G-395A and their influence on soluble α-Klotho levels in essential hypertension (EH) patients in Indian population. One-hundred-twelve EH patients and age, BMI-matched 103 normotensive controls were recruited. Soluble α-Klotho levels were measured by Enzyme-Linked-Immunosorbent-Assay. Genotyping of single nucleotide polymorphisms (SNP) was performed using TaqMan assays. Soluble α-Klotho levels in patients were significantly lower (26.9%,p=0.001) than controls. ROC curve analysis for soluble α-Klotho showed a cut-off of 580pg/ml with AUC 0.798(SE=0.031, 95%CI=0.737-0.859). Genotype distributions for both polymorphisms did not differ between the groups. Distribution of soluble α-Klotho levels in both groups did not vary across KL-VS genotypes. In patient group, GA and AA genotypes of G-395A polymorphism were associated with significantly lower (15.4%,p=0.014) soluble α-Klotho levels compared to GG genotype. Soluble α-Klotho levels were significantly lower in EH patients as compared to healthy normotensive individuals.

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Author Biographies

Gauri Pathare, Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Center

Ms. Gauri Pathare, M.Sc.

Designation: Ph.D. Fellow, Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Centre, Court House, L. T. Road, Mumbai 400002, India.

Sunila Raju, Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Center

Ms. Sunila Raju, M.Sc.

Designation: Research Assistant, Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Centre, Court House, L. T. Road, Mumbai 400002, India.

Vinod Shah, Sir H.N. Reliance Foundation Hospital and Research Center

Dr. Vinod Shah, DM (Card.), MD (Med.), FISE

Designation: Interventional Cardiologist; Consultant, Department of Cardiology, Sir H.N. Reliance Foundation Hospital and Research Center, Raja Ram Mohan Roy Road, Mumbai 400004, India

Manoj Mashru, Sir H.N. Reliance Foundation Hospital and Research Center

Dr. Manoj Mashru, MD (Med), DM (Card)

Designation: Interventional Cardiologist; Mentor, Department of Cardiology,

Sir H.N. Reliance Foundation Hospital and Research Center, Raja Ram Mohan Roy Road, Mumbai 400004, India

Kavita Shalia, Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Center

Dr. Kavita Shalia, Ph.D.

Designation: Senior Scientist, Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Centre, Court House, L. T. Road, Mumbai 400002, India.

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Published
2018-12-08
How to Cite
Pathare, G., Raju, S., Shah, V., Mashru, M., & Shalia, K. (2018). Association of Soluble α-Klotho and Klotho Polymorphisms (KL-VS and G-395A) with Essential Hypertension in Indian Population. International Journal of Biomedical and Advance Research, 9(12), 392-399. https://doi.org/10.7439/ijbar.v9i12.4969
Section
Original Research Articles