Phytochemical analysis, antimicrobial activity and identification of phytoconstituents in Gleichenia pectinata (Willd.) C. Presl.

  • Abiola Femi-Adepoju Kwara State University
  • Paul O. Fatoba Department of Plant Biology, University of Ilorin, Ilorin
  • Adeyinka Adepoju, Dr. wesley University of technology, Ondo
  • Abimbola P. Oluyori Department of Physical Sciences, Landmark University, Omu-Aran, Kwara State
Keywords: ferns, Gleichenia pectinata, Chromatography, Fractionation, Antimicrobial

Abstract

Probing into the bioactive chemical composition of ferns may lead to a validated incorporation into drug manufacture, though they belong to the unpopular group of plants. This present study aimed at investigating the phytoconstituents and antimicrobial potency of the raw methanolic extract and the active chromatographic fractions of Gleichenia pectinata. The phytochemical investigation was done via chemical and instrumental (GC-MS) method. The raw methanolic extract of G. pectinata contains important phytochemicals such as phenolics, terpenoids, anthraquinone, tannin, alkaloid, saponin, reducing sugar, protein and flavonoids. The minimum inhibitory concentration of the raw methanolic extract against the clinical microbial strains used was 25mg/ml against (Pseudomonas aeruginosa and Klebsiella pneumoniae). At this concentration, some G.pectinata chromatographic fractions exhibited significant antibacterial and antifungal activities. Pseudomonas aeruginosa and Klebsiella pneumoniae were the most susceptible of the test microorganisms while Candida albicans was the least susceptible.

Downloads

Download data is not yet available.

References

Levy, S. B. Drug Resistance: The New Apocalypse (special issue). Trends Microbiol. 1994; 2: 341–425.

Farina, M., Preeti, B. and Neelam, P. Phytochemical evaluation, antimicrobial activity, and determination of bioactive components from leaves of Aegle marmelos. BioMed Research International 2014; Article ID 497606, 11.

Lis-Balchin, M. and Deans S. Bioactivity of selected plant essential oils against Listeria monocytogenes. Journal of Applied Microbiology. 1997; 82 (6): 759–762.

Asakawa, Y. Agnieszka, L. and Fumihiro, N. Phytochemical and biological studies of bryophytes. J. phytochem. 2013; 91: 52-80.

Banerjee R. D. and Sen S. P. Antibiotic activity of pteridophytes. Econ. Bot. 1980; 34: 284–298.

Chen, E.W., Lim, Y. Y. and Chew, Y. L. Antioxidant activity of Camellia sinensis leaves and tea from a lowland plantation in Malaysia. J Food Chem. 2007; 102: 1214-1222.

Femi-Adepoju, A.G., Adepoju, A.O. and Ogunkunle, A.T. Assessment of fungicidal and fungistatic properties of some nigerian moss plants. Global J. Biol. Agric. Health. Sci. 2014; 3(1):126-131.

Pradeep, P., Leena, P. and Achaleshwar B. In vitro antibacterial activity of fronds (leaves) of some important pteridophytes. Journal or Microbiology and Antimicrobials, 2010; 2(2): 19-22.

Maher, O., Mohamad, S., Mohammad, A., Enas, A., Hanee, A., Maisa, A., Jafar, E. and Ismael, O. Antimicrobial activity of crude extracts of some plant leaves. Research Journal of Microbiology 2012; 7: 59-67.

NCCLS (National Committee for Clinical Laboratory Standards) Approved Standard. (2002). NCCLS M38-A. Villanova, PA, USA.

Madigan, M. Brock Biology of Microorganisms. San Francisco: Pearson/Benjamin Cummings. 2009: pp. 1152.

Sofowora, E.A. Medicinal Plants and Traditional Medicine in West Africa. Second ed, John Willey and Sons Ltd. New York, 1993; pp. 289.

Okwu, D. E. Evaluation of the chemical composition of indigenous Spices and flavouring agents. Global J. Appl. Sci. 2001; 7(3): 455-459.

Padmaja, G. Evaluation of techniques to reduce assayable tannin and cyanide in cassava leaves. J Agric. Food Chem. 1989; 37: 712-716.

Chan, E. W., Lim, Y. Y., Chew, Y. L. Antioxidant activity of Camellia sinensis leaves and tea from a lowland plantation in Malaysia. J Food Chem. 2007; 102: 1214-1222.

Kale, A., Gaikwad, S. and Mundhe, K. Quantification of phenolics and flavonoids by spectrophotometer from Juglans regia. Int J Pharm Biol Sci. 2010; 1: 1-4.

Singh, D., Srivastva, B. and Sahu, A. Spectrophotometric determination of Rauwolfia alkaloids, estimation of reserpine in pharmaceuticals. Anal Sci. 2004; 20: 571-573.

Carcia, F., Pivel, J. P., Guerrero, A., Brieva, A., Martinez-Alvazar, M. P., Caamano-Somoza, M. and Conzlez, S. Phenolic components and antioxidant activity of Fernblock, an aqueous extract of the aerial parts of the fern Polypodium leucotomos. Methods Find Exp. Clin. Pharmacol. 2006; 28: 157–160.

McCutcheon, A. R., Roberts T. E., Gibbons E., Ellis S. M., Babiuk L. A., Hancock R. E. and Towers, G. H. Antiviral screening of British Columbian medicinal plants. J. Ethnopharmacol. 1995; 49: 101–110.

Liu, B., Diaz, F., Bohlin, L. and Vasange, M. Quantitative determination of anti-inflammatory principles in some Polypodium species as a basis for standardization. Phytomedicine. 1998; 5: 1487–194.

Parihar, P. and Bohra, A. Screening of some ferns for their antimicrobial activity against Salmonella typhi. Adv. Plant Sci., 2002; 15: 365–368.

Herin, D.S., John, A. D. and Benjamin, P. J. Qualitative and quantitative analysis of phyto-chemicals in five pteris species. International Journal of Pharmacy and Pharmaceutical Sciences. 2013; 5(1).

CLSI (Clinical and Laboratory Standards Institute). (2014). Performance Standards for Antimicrobial Susceptibility Testing. twenty-fourth informational supplement.

Ojo, O.O. Ajayi, A.O. and Anibijuwon, I.I. Antibacterial potency of methanol extracts of lower plants. J Zhejiang Univ Sci B. 2007. 8(3): 189–191.

Samir, K.P. Study of antimicrobial activity of different plant parts of ferns. Ind J Biol Stud Res. 2013; 2(2): 134-138.

Oluyori A.P., Shaw A.K., Rastogi P., Sammajay R., Olubunmi A., Olatunji G.A. and Fabiyi O.A. Natural antifungal compounds from the peels of Ipomoea batatas. Nat. Prod. Res. 2015; 30(18): 2125-2129.

Adelani-Akande T.A., Ajiba L.C., Dahunsi S.O., Oluyori A.P. Antibacterial activity of watermelon (Citrullus lanatus) seed against selected microorganisms. Afr. J. Biotechnol. 2015; 14 (14): 1224-1229.

Oluyori, A.P., Arun, K.S., Olatunji, G.A, Preeti, R., Sanjeev, M., Dipak, D, Ashish A. Sama A., and Puli S.R. Sweet Potato Peels and Cancer Prevention. Nutrition and Cancer – An International Journal. 2016; 68(8): 1330-1337.

Moorthy, V. and Boominathan, M. The antimicrobial activities of crude extracts and fraction of Psidium guajava and Azadirachta indica against Staphylococcus aureus in chronic disease affected patients. International Journal of Universal Pharmacy and Life Sciences. 2011; 1(2): 2249–6793.

Turcotte, P. and Saheb, S. A. Antimicrobial activity of phenolic antioxidants. Canadian Journal of Microbiology. 1978; 24(11): 1306–1320.

Togashi, N., Shiraishi, A., Nishizaka, M. et al. Antibacterial activity of long-chain fatty alcohols against Staphylococcus aureus. Molecules, 2007; 12(2): 139–148.

Surender S., Vinod N., Sweety J., Gupta Y. K. Evaluation of anti-inflammatory activity of plant lipids containing α- linolenic acid. Indian Journal of Experimental Biology 2008; 46(6): 453–456.
Published
2018-12-08
How to Cite
Femi-Adepoju, A., Fatoba, P., Adepoju, A., & Oluyori, A. (2018). Phytochemical analysis, antimicrobial activity and identification of phytoconstituents in Gleichenia pectinata (Willd.) C. Presl. International Journal of Biomedical and Advance Research, 9(12), 400-406. https://doi.org/10.7439/ijbar.v9i12.4974
Section
Original Research Articles