In vitro Anti-drepanocytary (anti-sickle cell anemia) and Membrane Stability Potential of Mishenland Polyherbal Extract on Sickle Red Blood Cells

  • Oyenike Musiliu. A. Department of Biomedical Science, Ladoke Akintola University of Technology, Osogbo, Osun State, Nigeria
  • Akpan Helen. B. Department of Anatomy, Olabisi Onabanjo University, Ago-Iwoye, Ogun State
  • Otulana Jubril. O. Department of Anatomy, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria
  • Adefule Adebayo. K. Department of Anatomy, Olabisi Onabanjo University, Ago-Iwoye, Ogun State
  • Adedokun Kamoru. A. Department of Oral and Maxillofacial Pathology, Saud University Medical City, Riyadh http://orcid.org/0000-0001-9833-3011
  • Suleiman Ibrahim. E. Department of Chemical Pathology, University of Ilorin Teaching Hospital, Ilorin, Kwara State
  • Ojokuku Hammed. O. Department of Biomedical Science, Ladoke Akintola University of Technology, Osogbo. Osun State
  • Oluogun Waheed. A. Department of Morbid Anatomy and Histopathology, Ladoke Akintola University of Technology Teaching Hospital, Osogbo, Osun State
  • Muhibi Musa. A. Department of Haematology and Blood Transfusion, Ladoke Akintola University of Technology Teaching Hospital, Osogbo, Osun State
  • Tijani Busira. A. Department of Haematology and Blood Transfusion, Ladoke Akintola University of Technology Teaching Hospital, Osogbo, Osun State
Keywords: Drepanocytosis, erythrocyte membrane stability, in vitro antisickling, Mishenland polyherbal formula, phytochemical screening, sickle cell anaemia

Abstract

Background: Drepanocytosis (sickle cell anaemia) is a genetic disorder that causes stiff, rod-like sickle-shaped haemoglobin in red blood cells (RBCs) and consequently poses serious health complications. We investigated in vitro anti-sickling potential of a novel polyherbal formula (MPF). 

Methods: Sickling of RBCs induced with 2%sodium metabisulphite (MBS) was followed by treatment with MPF mixtures in different saline concentrations (7mg/ml, 9mg/ml, 14mg/ml and 28mg/ml). The red cell morphology was examined microscopically. Percentage sickling was quantified 30-minute interval at 370C for 2-hours. Effect of the MPF on membrane stability of RBCs was analyzed using osmotic fragility testing.

Results: Qualitative phytochemical screening demonstrated the presence of some secondary metabolites namely; alkaloids, glycosides, phenols, saponin, tannin and terpenoids. Sickling of RBCs induced by MBS was inhibited by MPF. This antisickling effect was directly proportional to concentration of the MPF, dose-dependently. There was significant difference (P<0.05) between MPF-treated and untreated sickle cell counts. Osmotic fragility curves obtained from MPF-treated RBCs showed leftward shift against the untreated control indicative of increased membrane stabilization and/or haemolytic resistance.

Conclusion: MPF demonstrated significant anti-sickling and erythrocyte membrane stability properties. These effects under hypoxia signify a promising effect of the bioactive components as probable drug candidates against sickling of red cells.

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Author Biography

Adedokun Kamoru. A., Department of Oral and Maxillofacial Pathology, Saud University Medical City, Riyadh

Mr ADEDOKUN K. Ademola is a graduate of Medical Laboratory Science with Second Class Upper Division. He also holds Higher National Diploma (HND) in Microbiology and Master of Science (M.Sc.) in Chemical Pathology, all from reputable institutions in Nigeria. He obtained certificates in different areas of epidemiology, mostly from USAID (in collaboration with John Hopkins Bloomberg School of Public Health, USA) and another from University of Washington, USA.

Currently, he works at the department of Oral Pathology in King Saud University Medical City, Saudi Arabia.
He is a member/registered professional of many reputable societies/health councils such as Association of Medical Laboratory Science Council of Nigeria (MLSCN); Saudi Commission for Health Specialties (SCFHS), Australasian Immunohistochemistry Society (AIS), Australia, among others.
He is a Medical Scientist with great passion and drive for laboratory medicine. His future aspirations involve research and teaching molecular oncology. He has worked in different clinical settings ranging from government to private organizations and equally both at national and international levels. He is specializing in oncology, God willing.

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Published
2018-11-26
How to Cite
1.
A. O, B. A, O. O, K. A, A. A, E. S, O. O, A. O, A. M, A. T. In vitro Anti-drepanocytary (anti-sickle cell anemia) and Membrane Stability Potential of Mishenland Polyherbal Extract on Sickle Red Blood Cells. IJBR [Internet]. 26Nov.2018 [cited 16Dec.2018];9(11):373-9. Available from: https://ssjournals.com/index.php/ijbr/article/view/4928
Section
Original Research Articles