Expression of HIF-1, VEGF-β and Caspase-3 in Myocardium of Rats Subjected to Hypoxic-Hyperglycemic Condition
Introduction: Studies of the protective effects of insulin on endothelial impairments are still very limited.
Aim: This study is intended to find out the effect of insulin injection on expression of HIF-1, VEGF-β, and caspase-3 in myocardial tissues of rats subjected to hypoxic-hyperglycemic condition.
Materials and methods: Wistar rats (n=30) were subjected to hyperglycemic by alloxan injection and be hypoxic by exposuring the animal to a normal and low oxygen tension alternatingly. The test animals then grouped into two. The first group was treated with insulin NPH, the second one was not.
Results: Results showed that expression of HIF-1 and caspase-3 did not significantly affected by insulin treatments. However, expression of VEGF-β was siginificantly increased by insulit injection.
Conclusion: In conclussion, insulin has pontential to be used as a ptotective agent against endothelial impairments in rats experienced hypoxia and hyperglycemia.
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