Expression of HIF-1, VEGF-β and Caspase-3 in Myocardium of Rats Subjected to Hypoxic-Hyperglycemic Condition

  • Toni Prasetia Faculty of Medicine, Malahayati University
  • Desy Hermawan Biomedical Sciences, Faculty of Medicine, Malahayati University, Bandar Lampung
  • Yan wirasti Department of Pathological Anatomy, Faculty of Medicine, Andalas University, Padang
  • Eva Decroli Internal Medicine, Faculty of Medicine, Andalas University, Padang
  • Masrul Masrul Clinical Nutrition, Faculty of Medicine, Andalas University, Padang
  • Mohammad Kanedi Department of Biology, Faculty of Mathematics and Sciences, University of Lampung, Bandar Lampung


Introduction: Studies of the protective effects of insulin on endothelial impairments are still very limited. Aim: This study is intended to find out the effect of insulin injection on expression of HIF-1, VEGF-β, and caspase-3 in myocardial tissues of rats subjected to hypoxic-hyperglycemic condition. Materials and methods: Wistar rats (n=30) were subjected to hyperglycemic by alloxan injection and be hypoxic by exposuring the animal to a normal and low oxygen tension alternatingly. The test animals then grouped into two. The first group was treated with insulin NPH, the second one was not. Results: Results showed that expression of HIF-1 and caspase-3 did not significantly affected by insulin treatments. However, expression of VEGF-β was siginificantly increased by insulit injection. Conclusion: In conclussion, insulin has pontential to be used as a ptotective agent against endothelial impairments in rats experienced hypoxia and hyperglycemia. Used for easily cloning the properly namespaced rect


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Prasetia T, Hermawan D, wirasti Y, Decroli E, Masrul M, Kanedi M. Expression of HIF-1, VEGF-β and Caspase-3 in Myocardium of Rats Subjected to Hypoxic-Hyperglycemic Condition. IJBR [Internet]. 30Dec.2018 [cited 24Mar.2019];9(12):403-8. Available from:
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