Evaluation of Analgesic and Anti-Inflammatory Activities of hydro-alcoholic leaf extract of Hypericum revolutum in Mice
Background: Despite the progress that has been made in the past for the development of pain and inflammation therapy, there is a need for effective and potent medicinal agent with lower side effects. The aim of this study was to evaluate the analgesic and anti-inflammatory activities of 80% methanol leaf extract of Hypericum revolutum in mice.
Method: After extraction of the crude using 80% methanol, Hypericum revolutum extract was evaluated for analgesic and anti-inflammatory activity in hot plate test, acetic-acid induced writhing and carrageenan induced paw edema models. Extract was evaluated at 100, 200 and 400 mg/kg doses. The reference control groups were treated with morphine 5mg/kg or indomethacin 10mg/kg while vehicle, distilled water (10 ml/kg), treated animals were grouped as negative controls.
Result: In the hot plate method, all doses of the extract and the standard drug morphine significantly prolonged the latency time in the hot plate method (p<0.05 or p<0.01 or p<0.001) when compared to the negative control throughout the observation time. Greater increase in latency was observed at the 120 minute and greater on the other time intervals with 200mg/kg of the extract with a significant value (p<0.001). Similarly, all test doses of the extract significantly (p<0.05) reduced the acetic acid induced writhing in mice. The extract of HR produced a significant analgesic activity with 62.4, 47.1 and 55.3% inhibition of number of writhing at 100, 200 and 400 mg/kg dose levels, respectively although the inhibition was greater for the standard.
Moreover, for carrageenan induced paw edema test treatment with the extract (100, 200, and 400mg/kg) and the standard have shown significance (p<0.01 or p<0.001) suppression of edema at all-time points as compared to controls.
Conclusion: The 80% hydro-methanolic extract of H. revolutum exhibited analgesic and anti-inflammatory effects in mice models.
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