Identification of nonvolatile phenolic acids and flavonoids with antioxidant activity in Micromeria barbata extract by RF-HPLC

  • Youssef Bakkour Laboratory of Applied Chemistry, Faculty of Science III, Lebanese University, P. O. Box 826, Tripoli,
  • Noha Ghanem Laboratory of Applied Chemistry, Faculty of Science III, Lebanese University, P. O. Box 826, Tripoli,
  • Bassem El Hamaoui Laboratory of Applied Chemistry, Faculty of Science III, Lebanese University, P. O. Box 826, Tripoli,
  • Nassim El-Achi Department of chemistry, Faculty of Science, University of Balamand, P.O. Box 100, El-Kurah,
  • Saer Alwan Laboratory of Pharmacognosy, Faculty of Public Health, University Of Jinan, P. O. Box 818, Tripoli,
  • Fatima Houmaisi Laboratory of Applied Chemistry, Faculty of Science III, Lebanese University, P. O. Box 826, Tripoli,
  • John Hanna EL-NAKAT Department of chemistry, Faculty of Science, University of Balamand, P.O. Box 100, El-Kurah,
  • Fawaz El-Omara Laboratory of Applied Chemistry, Faculty of Science III, Lebanese University, P. O. Box 826, Tripoli
Keywords: RP-HPLC, retention time, calibration curve, phenolic acids, flavonoids, Micromeria barbata, Antioxidant activity

Abstract

An effective RF-HPLC method was performed for the determination and purification of nonvolatile mixture of phenolic acid and flavonoids contents in Micromeria barbata ethanolic extract. The HPLC was done on Ascentis RF-Amide at 25C using a mixture of diluted orthophosphoric aqueous acid (0.085%) (A) acetonitrile (B). A gradient elution was conducted starting at 85% and ending with 15% of A after 60 minutes. Identification of phenolic acids and flavonoids was done by comparing their retention times to those of stock standard compounds. Aluminum chloride and FolinCiocalteau spectrophotometry analysis was used for quantification. It was found that the ethanolic extract inhibits the DPPH radical with an IC50 (26.74?g/ml) which was significantly less than that of BHT (91.40 ?g/ml).

Downloads

Download data is not yet available.
Published
2014-12-30
How to Cite
Bakkour, Y., Ghanem, N., Hamaoui, B., El-Achi, N., Alwan, S., Houmaisi, F., EL-NAKAT, J., & El-Omara, F. (2014). Identification of nonvolatile phenolic acids and flavonoids with antioxidant activity in Micromeria barbata extract by RF-HPLC. International Journal of Pharmaceutical Chemistry, 4(4), 142-145. https://doi.org/10.7439/ijpc.v4i4.99
Section
Research Articles