Application of Validated HPLC Method for Degradation Study of Vildagliptin and Metformin HCl
AbstractA novel and simple reverse phase liquid chromatographic method has been established for the determination of Vildagliptin and Metformin HCl and studies its degradation pattern in pharmaceutical dosage forms. Vildagliptin and Metformin HCl is used to control Type 2 Diabetes. The proposed work was performed on Younglin (S.K) isocratic System UV Detector C18 column (150 mm 4.6 mm). A mixture of Potassium Phosphate, mobile phase in this method with flow rate 0.7 ml/min (UV detection at 203 nm) and the method was validated as per ICH guidelines. Forced degradation studies were performed by exposing the drug Vildagliptin and Metformin HCl to acidic, alkaline, oxidation and thermal stress degradations. The proposed RP-HPLC method was found to be robust and specific and this method is suitable for the assay of pharmaceutical dosage forms as well as kinetic studies.
Copyright (c) 2017 International Journal of Pharmaceutical Chemistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).
- The author must submit Copyright form After acceptance of the article.