A Novel and Rapid Method for Formulation and Simultaneous Evaluation of Polyherbal Tablet for Hypothyroidism by HPTLC
Herbal Pharma industry is rapidly expanding its demand in national as well as international market. Due to lack of pharmaceutical and analytical validation of herbal products, quality of herbal products differs from batch to batch. Pharmaceutical and analytical validation can be achieved, if the herbal products are evaluated and analyzed using both herbal as well as modern techniques of standardization; in process and after preparation of finished product. A fast, rapid, sensitive and stability indicating high-performance thin-layer chromatographic (HPTLC) method is developed and validated for qualitative estimation of Patol (Trichosanthes dioica), Indrajav (Holarrhena antidysenterica), Kanchnar (Bauhinia variegate), Shigru (Moringa oleifera), Varun Chal (Crataeva nurvala) and Trikatu (Piper longum, Piper nigrum and Zingiber officinalis) simultaneously in pharmaceutical herbal formulation (Tablet). Chromatographic separation of the herbs is performed on aluminium backed plates coated with silica gel 60F254 as the stationary phase and the solvent system consisted of n-Hexane: n-Butanol: Toluene: Ethyl Acetate (5:5:3:2). Samples were applied with Linomat V under nitrogen gas flow. The densitometric analysis was performed CAMAG TLC scanner 4 at 300nm. All the three samples prepared by this method shows identical characteristics and analytical parameters does not show significant difference. Also the observations of analytical study show similarity to those parameters which are already set in API for this drug.
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