Investigation of cerebroprotective effect of Bixa orellana on sodium nitrite induced hypoxic neurotoxicity

  • Syeda Nishat Fathima Assistant Professor, Jayamukhi College of Pharmacy
  • K. Lakshman Kumar Associate Professor, Department of Pharmacognosy, Trinity College of Pharmacy, Karimnagar, Telangana
  • K. Shailusha Department of Pharmacology, Jayamukhi College of Pharmacy, Narsampet, Warangal, Telangana
Keywords: Cerebroprotective, Hypoxia, Bixa orellana


The present study was carried out to evaluate the cerebroprotective effects of ethanolic extract of Bixa orellana leaves in hypoxic neurotoxicity induced rats. Hypoxia was induced by treatment of rats with sodium nitrite in drinking water for 30 days. Bixa orellana attenuated cognitive deficits in sodium nitrite induced rats which was studied by Morris water maze test.  The extract restored the decreased levels of dopamine and increased levels of acetylcholinesterases and glutamate to the normal levels which can be compared with standard Pyritinol. The present study reveals that the ethanolic extract of Bixa orellana leaves confers cerebroprotection against sodium nitrite induced hypoxic neurotoxicity in rats.


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Shahid-ul-Islam, Luqman JR, Faqeer M. Phytochemistry, biological activities and potential of annatto in natural colorant production for industrial applications A review. Journal of Advanced Research. 2016; 7: 499514.

Radhika B, Begum N, Srisailam K. Pharmacognostic and Preliminary Phytochemical Evaluation of the leaves of Bixa orellana. Pharmacognosy Journal. 2010; 2: 132136.

Dennis DR, Rafael AE, Chien-Chang S, Consolacion YR. A bioactive sesquiterpene from Bixa orellana. J Nat Med (2011) 65:206211.

Prathima D, Sujitha A, Usha R. Phytochemical Screening and Antimicrobial Activity of Bixa Orellana Linn. International Journal of Pharmacognosy and Phytochemical Research. 2016; 8(7): 1078-1082

Garcia VMN, Gonzalez A, Fuentes M, Aviles M, Rios MY, Zepeda G, et al. Antifungal activities of nine traditional Mexican medicinal plants. J Ethnopharmacol 2003; 87:858.

Cceres A, Menndez H, Mndez E, Cohobn E, Samayoa BE, Jauregui E, Peralta E, Carrillo G. Antigonorrhoeal activity of plants used in Guatemala for the treatment of sexually transmitted diseases. J Ethnopharmacol 1995 ;48:858.

Monzote L, Garc?a M, Scull R, Cuellar A, Setzer WN. Antileishmanial activity of the essential oil from Bixa orellana. Phytother Res.2014; 28(5):7538.

Zhai B, Clark J, Ling T, Connelly M, Medina-Bolivar F, Rivas F. Antimalarial evaluation of the chemical constituents of hairyroot culture of Bixa orellana L.. Molecules. 2014; 19:75666.

Kuppusamy AK, Puliyath J, Muthusamy U, Thirumalaisamy S, Varadharajan S, Arumugam M. Cardioprotective activity of Bixa orellana L. on isoproterenol induced myocardial necrosis in rats. Journal of Pharmacy Research. 2012; 5(4):1930.

Zuraini A, Somchit MN, Hamid RA, Sukradi S, Fazira AJSE, Yong YK, et al. Inhibitions of acute and chronic inflammations by Bixa orellana leaves extract. Planta Med. 2007; 73:76.

Huaman O, Sandoval M, Arnao I, Be jar E. Antiulcer effect of lyophilized hydroalcoholic extract of Bixa orellana (annatto) leaves in rats. An Fac Med. 2009; 70:97102.

Ahsan R, Islam KM, Musaddik A, Haque E. Hepatoprotective activity of methanol extract of some medicinal plants against carbon tetrachloride induced hepatotoxicity in albino rats. Global J Pharmacol. 2009; 3:11622.

Radhika B, Begum N, Srisailam K, Reddy VM. Diuretic activity of Bixa orellanna Linn. leaf extracts. Ind. J Nat Prod Res. 2010; 1(3):3535.

Quanico JP, Amor EC, Perez GG. Analgesic and hypoglycemic activities of Bixa orellana, Kyllinga monocephala and Luffa acutangula. Philippine J Sci. 2008; 137:6976.

Shilpi JA, Taufiq-Ur-Rahman M, Uddin SJ, Alam MS, Sadhu SK, Seidel V. Preliminary pharmacological screening of Bixa orellana L. leaves. J Ethnopharmacol. 2006; 108:26471.

Reddy MK, Alexander-Lindo RL, Nair MG. Relative inhibition of lipid peroxidation, cyclooxygenase enzymes, and human tumor cell proliferation by natural food colors. J Agric Food Chem. 2005; 53:926873.

Abayomi M, Adebayo AS, Bennett D, Porter R, Shelly- Campbell J. In vitro antioxidant activity of Bixa orellana (Annatto) seed extract. J Appl Pharm Sci. 2014; 4:101106.

Chiste RC, Mercadante AZ, Gomes A, Fernandes E, Lima JLFC, Bragagnolo N. In vitro scavenging capacity of annatto seed extracts against reactive oxygen and nitrogen species. Food Chem. 2011; 127:419426.

OECD/ OCDE, OECD Guideline for Testing of Chemicals, Acute Oral Toxicity- Acute Toxicity Class Method, 423. Adopted 17th December, 2001. [Cited on 10 October 2017]. Available from:

Dhas J, Dhas DJ, Venkataraman S, Ernest A, Bright SE. The protective effects of Cyperus rotundus on behavior and cognitive function in a rat model of hypoxia injury, Pharmaceutical Biology. 2014; 52:12, 1558-1569.

Morris R. Developments of a water maze procedure for studying spatial learning in the rat. J Neurosci Methods. 1984; 11: 47-60.

Schlumpf M, Lichtensteiger W, Langemann H, Waser PG, Hefti F. A fluorimetric micromethod for the simultaneous determination of serotonin, noradrenaline and dopamine in milligram amounts of brain tissue. Biochemical Pharmacology. 1974; 23:2337-2346.

George LE, Diane CK, Valentino A, Robert MF. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology. 1961. 7(2); 88-95

Bernt E, Bergmeyer HU. L-glutamate UV assay with glutamate dehydrogenase and NAD. In: Methods of enzymatic analysis. 2nd ed. New York, Academic Press, 1965; 1704-1708.

Seyfried, CA. Neurochemical effects of pyritinol and their relevance for the treatment of brain function deficits in old age. Drug Dev. Res. 1989; 18: 19.

Gluhcheva Y, Ivanov I, Petrova E, Pavlova E, Vladov I. Sodium nitrite-induced hematological and hemorheological changes in Rats. Series on Biomechanics. 2015; 27(3-4): 53-58

Finla C, Amee K, Cheramadathikudyil SP. Acetylcholine esterase activity and behavioral response in hypoxia induced neonatal rats: Effect of glucose, oxygen and epinephrine supplementation. Brain and Cognition. 2008; 68(1):59-66

Sangu M, Panchanan M, Soumya P, Preeti S, Alpesh KS, Shashi BS, Dipti P, Govindasamy I. Role of cholinergic markers on memory function of rats exposed to hypobaric hypoxia. European Journal of Pharmacology. 2011; 672(1-3): 96-105.

Wustmann C, Fisher HD, Schmidt J. Inhibitory effects ofbhypoxia on dopamine release. Acta Biol Med Germ. 1982; 41(6):571-4.

Kumar GK. Hypoxia. 3. Hypoxia and neurotransmitter synthesis. American Journal of Physiology - Cell Physiology. 2011; 300(4):C743-C751.

How to Cite
Fathima, S., Lakshman Kumar, K., & Shailusha, K. (2018). Investigation of cerebroprotective effect of Bixa orellana on sodium nitrite induced hypoxic neurotoxicity. International Journal of Phytopharmacy, 8(1), 01-05.
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