Cross-cytotoxicity study of antimalarial drugs and traditional medicinal plants on Renal Cells
AbstractObjective: The association of Artemisinin based combination therapy with herbal medicine seems to be a factor favouring the occurrence of serious adverse effects, hepatitis disorders and acute renal failure in particular. The aim of this study was to evaluate the cross- toxicity of these associations on renal cells.
Methods: African green monkey kidney cells (VERO) were incubated with anti-malarial drugs and plant preparations. The products used were: Artesunate 100mg / amodiaquine 270mg (ASAQ) and artemether 80mg / lumefantrine 480mg (AL) , and Sida acuta and Enantia polycarpa at a concentration of 10 ?g / ml. After 5 days of incubation, a cell count was performed using a haemocytometer.
Results: ASAQ resulted in significant cell destruction compared to the control between Day2 (p <0.05) and Day5 (p <0.01) with a peak at day 3 (p <0.001). There was also a significant difference between Sida acuta and control on Day4 (p <0.05) and Day5 (p <0.001). Cell mortality was> 30% in the tubes treated with ASAQ and Sida acuta. Finally, cell destruction was statistically significant in tubes treated with the combination of antimalarial drugs and traditional plants compared to the control tube from Day2 (p <0.001).
Conclusion: The renal toxicity observed with ASAQ, Sida acuta and combination of antimalarial drugs and herbs, appears dose-dependent or cumulative. The mechanism of this renal toxicity should be better analysed in further studies.
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