Antiviral potential of mangiferin against poliovirus

  • Daniele Zendrini Rechenchoski Universidade Estadual de Londrina
  • Ligia Carla Faccin-Galhardi Universidade Estadual de Londrina
  • Arcelina Pacheco Cunha Universidade Federal do Ceará
  • Ngila Maria Pontes Silva Ricardo Universidade Federal do Ceará
  • Carlos Nozawa Universidade Estadual de Londrina
  • Rosa Elisa Carvalho Linhares Universidade Estadual de Londrina
Keywords: Antiviral, mangiferin, poliomyelitis, poliovirus

Abstract

Poliovirus (PV),an enterovirus of the Picornaviridae family, is the agent of poliomyelitis, a devastating neurologic disease that may result in paralysis and even death.Mangiferin is the major constituent of Mangifera indica and has multiple pharmacological activities, including antiviral. This study evaluated the activity of mangiferin against poliovirus type-1 (PV-1). The compound exhibited the 50% cytotoxic concentration (CC50) > 2000 μg/mL in HEp-2 cell cultures, by the dimethylthiazolyl-diphenyltetrazolium bromide method (MTT). The 50% inhibitory concentration (IC50) was of 53.5 μg/mL, determined by the plaque reduction assay (PRA) with selectivity index (SI) > 37.4. We investigate the possible mechanism of action of mangiferin and a maximum inhibition of PV was found for virucidal and inhibition of adsorption assays, at all tested concentrations. The inhibition of viral protein synthesis was also demonstrated by immunofluorescence assay (IFA).These results suggested that mangiferin is an attractive candidate for the control of PV infection.

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Author Biographies

Daniele Zendrini Rechenchoski, Universidade Estadual de Londrina

Departamento de Microbiologia

 

Ligia Carla Faccin-Galhardi, Universidade Estadual de Londrina
Departamento de Microbiologia
Arcelina Pacheco Cunha, Universidade Federal do Ceará
Departamento de Química Orgânica e Inorgânica
Ngila Maria Pontes Silva Ricardo, Universidade Federal do Ceará
Departamento de Química Orgânica e Inorgânica
Carlos Nozawa, Universidade Estadual de Londrina
Departamento de Microbiologia
Rosa Elisa Carvalho Linhares, Universidade Estadual de Londrina
Departamento de Microbiologia

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Published
2018-04-24
Section
Research Articles