Objectives: To evaluate association of genetic polymorphisms in GSTM1, T1, and P1 genes with cervical cancer (CC) susceptibility and effect of passive smoking.
Methods: The polymorphisms in GSTM1 and T1 were analyzed by multiplex polymerase chain reaction (mPCR) and GSTP1 by PCR – Restriction Fragment Length Polymorphism (PCR-RFLP) in 150 CC cases and 165 healthy controls.
Results: CC cases showed significant association with marriage age, parity and hemoglobin level (p<0.0001). GSTM1 (-/-) genotype was prominent among cases (30.0 %) whereas on combined analysis, GSTM1(-/-) / T1(-/-) and GSTM1(-/-) / P1(A/G or G/G) genotypes showed 2.75 and 2.98 times higher susceptibility to CC respectively. Triple combinations of GSTM1 (-/-), T1 (+/+) and P1 (A/G or G/G) genotypes showed significant association (P=0.02) with a 3.04 times risk of developing CC. Further analysis of cervical cancer histological subtypes such as squamous cell carcinoma and adenocarcinoma revealed an association of GSTM1 (-/-), GSTP1 A/G and G/G genotypes in adenocarcinoma cases alone (4.01, 2.25 and 2.40 folds respectively). Out of all the CC cases, passive smokers with GSTM1 (-/-) and GSTP1 (G/G) genotypes showed 4.19 and 2.88 times respectively of increased risk of developing the disease.
Conclusions: This study confirms the possible interactions between different GST polymorphisms and passive smoking in cervical cancer cases from North India.
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