Objective: The objective of the present study was to investigate the cardioprotective effects of methanolic extract of leaves of Andrographis paniculata against Isoproterenol-induced myocardial infarction (MI) in rats. Method: The rats were divided into five experimental groups viz., Normal control, ISO-treated (Disease control), Propranolol (10 mg/kg + ISO), Andrographis paniculata (100 mg/kg +ISO) and Andrographis paniculata (200 mg/kg + ISO). Myocardial infarction in rats was induced by the administration of isoproterenol at a dose of 85mg/kg i.p., the rats in group IV and group V were pretreated with methanolic extract of Andrographis paniculata in the dose of 100mg/kg b.w. and 200mg/kg b.w. through oral route. Cardiac marker enzymes, lipid profile and antioxidant enzymes as biomarker of cardiotoxicity were determined in experimental animals. Result: Animals treated with flavonoid of leaves of Andrographis paniculata showed significant decrease in LDL-Cholesterol, total cholesterol, Triglycerides, AST, ALT, ALP, antioxidant enzymes viz., superoxide dismutase, catalase LPO and increase in HDL-Cholesterol and further was confirmed by histopathological study. Conclusion: The results of the study demonstrate that Andrographis paniculata strongly protected the myocardium against isoproterenol-induced infarction and suggest that the cardioprotective effects could be related to antioxidant activities.
Kubler W, Haass M. cardioprotection: definition, classification and fundamental principles. Heart 1996; 75:330-33.
Grundy SM, J Am Med Assoc 1986; 256: 849
Brown MS, Goldstein JL. Science v, 34, 232
Castelli WP, Garrison RJ, Wilson PWF, Abott RD, Kalousidan S, Kannel WB, J Am Med Assoc 1986; 256: 2835
Jarukamjorn K, Nemoto N. Pharmacological aspects of 11. Andrographispaniculata as health and its major diterpenoid constituent Andrographolide. J Health Sci 2008; 54: 370-81.
Tripathi R, Kamat JP. Free radical induced damages to rat liver 12. Subcellular organelles: inhibition by Andrographis paniculata extract. Indian J ExpBiol 2007; 45: 959-67.
Shen YC, Chen CF, Chiou WF. Andrographolide prevents 13. Oxygen radical production by human neutrophils: possible mechanism(s) involved in its anti-inflammatory effect. Br J Pharmacol 2002; 135: 399-406.
Puri A, Saxena R, Saxena RP, Saxena KC, Srivastava V, 14. Tandon JS. Immunostimulant agents from Andrographis paniculata. J Nat Prod 1993; 56: 995-9.
Zhang XF, Tan BK. Antihyperglycaemic and anti-oxidant 15. Properties of Andrographis paniculata in normal and diabetic rats. Clin Exp Pharmacol Physiol 2000; 27: 358-63.
Zhang CY, Tan BK. Mechanisms of cardiovascular activity 16. Of Andrographis paniculata in the anaesthetized rat. J Ethnopharmacol 1997; 56: 97-101.
Yoopan N, Thisoda P, Rangkadilok N, Sahasitiwat S, Pholphana 17. N, Ruchirawat S, et al. Cardiovascular effects of 14-deoxy-11, 12-didehydroandrographolide and Andrographis paniculata extracts. Planta Med 2007; 73: 503-11.
Zhang J, Yong-Fen Qi, Gengb B, Effect of relaxin on myocardial ischemia injury induced by isoproterenol. Peptides 2005; 26: 1632-1639.
Prince P, Suman S, Devik P T, Cardioprotective effect of
Cabaud P, Wroblewski F, Colorimetric measurement of lactic dehydrogenase activity of body fluid. Am J Clin Pathol 1958: 30: 234-236.
Aebi H, Bergmeyer HU, Methods of enzymatic analysis. New York: Academic Press Inc 1974: 673-685.
Marklund S, Marklund G, Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974; 47: 469-474.
Wilbur KM, Bernheim F, Shapiro OW. Determination of lipid peroxidation. Arch Biochem 1949; 24: 305-310.
Karthick M, Stanley Mainzen Prince P. Preventive effect of rutinon lipids, lipoproteins and ATPases in normal and isoproterenol-induced myocardial infarction in rats. J Biochem Mol Toxicol, 2007; 21(1):1-6.
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