Hyperlipidemia, Triton WR – 1339, Pandanus amaryllifolius, HMG – CoA reductase
The 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reductase is the key enzyme of cholesterol biosynthesis pathway in the liver. The present work aimed at investigating the hypolipidemic effect of total extract from Pandanus amaryllifolius Roxb. (PA) leaves through the inhibition of HMG-CoA reductase activity. PA extract showed a moderate in vitro inhibitory effect on activity of HMG-CoA reductase with IC50 value at range of 126.42 microg/ml as compared to that of atorvastatin about 7.77 ng/ml. In a mouse model of hyperlipidemia induced by a single intravenous injection of triton WR-1339, oral administration of PA extract significantly reduced total cholesterol as well as triglyceride levels as compared to hyperlipidemic mice. Furthermore, PA extract also inhibited HMG-CoA reductase activity with reduction of 32%, which were comparable to those of positive control, atorvastatin. In conclusion, PA extract clearly showed promising hypolipidemic effect by inhibiting HMG-CoA reductase activity.
Jamkhande P. G., Chandak P. G., Dhawale S. C., Barde S. R., Tidke P. S., Sakhare R. S. Therapeutic approaches to drug targets in atherosclerosis. Saudi Pharm. J. 2014; 22:179-190.
Stone J., Robinson G., Lichtenstein H., Noel Bairey Merz C., Blum B., Eckel H., et al. ACC/AHA Guildline on the Treatment of Blood Cholesterol to reduce Atheroscleotic Cardiovascular Risk in adults. J Am Coll Cardiol.2013; 63(25): 2889-2934.
DeBose-Boyd R. A. Feedback regulation of cholesterol synthesis: sterol-accelerated ubiquitination and degradation of HMG CoA reductase. Cell Res.2008; 18(6): 609-621.
Buerki S., Callmander M.W., Devey D.S., Chappell L., Gallaher T., Munzinger J., et al. Straightening out the screw pines: A first step in understanding relationships within Pandanaceae. Taxon.2012; 61(5): 1010-1020.
Golomb B.A., Evans M. A. Statin adverse effects: a review of the literature and evidence for a mitochondrial mechanism. The American Journal of Cardiovascular Drugs. 2008; 8(6):373â€“418.
Le Thi Ngoc Anh, Huynh Ngoc Trinh. Hypoglycemic effect and acute toxicity of total extract from Pandanus amaryllifolius leaves, Pharmaceutical Journal (Viet Nam), 2015; 475(55): 50-53.
Chiabchalard A., Nooron N. Antihyperglycemic effects of Pandanus amaryllifolius Roxb. leaf extract. Phcog Mag.2015; 11(41):117-122.
Nor F.M., Mohamed S., Idris N.A., Ismail R. Antioxidative Properties of Pandanus amaryllifolius Leaf Extracts in Accelerated Oxidation and Deep Frying Studies. Food Chemistry.2008; 110:319-327.
Chong H.Z., Yeap S.K., Rahmat A., Akim A.M., Alitheen N.B., Othman F., et al. In vitro evaluation of Pandanus amaryllifolius ethanol extract for induction of cell death on non-hormone dependent human breast adenocarcinoma MDA-MB-231 cell via apoptosis. BMC Complementary and Alternative Medicine. 2012; 12:134.
Ooi L.S.M., Sun S.S.M., Ooi V.E.C. Purification and Characterization of a New Antiviral Protein from the Leaves of Pandanus amaryllifolius (Pandanaceae).The International Journal of Biochemistry & Cell Biology. 2004; 36: 1440-1446.
Taylor & Francis. Chemical Constituents and Post-Harvest Prospects of Pandanus amaryllifolius Leaves: A Review. Food Reviews International. 2010; 26: 230-245.
Mastura W., Mohamad Azlan B.T. Extracts of the aerial roots from Pandanus amaryllifolius, Dissertation submitted in partial fulfilment of the requirements for the degree of Bachelor of Pharmacy (Hons.).Universiti Teknologi Mara.2010;7-9
Bertges L.C., C.A. Mourao Jr, J. B. Souza, V. A. C. Cardoso. Hyperlipidemia induced by Triton WR1339 (Tyloxapol) in Wistar rats. Rev Bras Cien Med Saude.2011; 1(1): 32-4.
Huynh Ngoc T., Nguyen Ngoc Q., Tran Thi Van A., Vo Phung N. Hypolipidemic Effect of Extracts from Abelmoschus esculentus L. (Malvaceae) on Tyloxapol-Induced Hyperlipidemia. Mah. Uni. Jour. of Pharm. Sci. 2008; 35(1-4):42-46.
Goldfarb S. Rapid increase in hepatic HMG CoA reductase activity and in vivo cholesterol synthesis after Triton WR 1339 injection. Journal of Lipid Research.1978; 19:489-494.
Kuroda M., Tanzawa K., Tsujita Y., Endo A. Mechanism for elevation of hepatic cholesterol synthesis and serum cholesterol levels in Triton WR- 1339-induced hyperlipidemia. Biochim. Biophys. Acta.1977; 489:119-125.
Patel H.D., Shah G.B., Trivedi V. Investigation of HMG-CoA reductase inhibitory activity of antihyperlipidemic herbal drugs study. Asian J. Exp. Biol. Sci.2011; 2(1):63-68.
Mukherjee P.K., Maiti K., Mukherjee K., Houghton P.J. Leads from Indian medicinal plants with hypoglycaemic potentials. Journal of Ethnopharmacology 2006; 106(1):1-28.
Mukherjee PK. Evidence-Based Validation of Herbal Medicine. Elsevier; 2015. p.385.
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